Rabbit Recombinant Monoclonal CXCR3 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free (AB274420), expandable thumbnail](https://content.abcam.com/products/images/cxcr3-antibody-epr23845-44-bsa-and-azide-free-ab274420--flow-cytometry-img126350.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free (AB274420)")
![Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free (AB274420), expandable thumbnail](https://content.abcam.com/products/images/cxcr3-antibody-epr23845-44-bsa-and-azide-free-ab274420--flow-cytometry-img439607.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free (AB274420)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Isoform 1. Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway (PubMed:12782716). Binds to CCL21. Probably promotes cell chemotaxis response. Upon activation by PF4, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Isoform 2. Receptor for the C-X-C chemokine CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the proliferation, survival and angiogenic activity of human microvascular endothelial cells (HMVEC) through a cAMP-mediated signaling pathway (PubMed:12782716). Does not promote cell chemotaxis respons. Interaction with CXCL4 or CXCL10 leads to activation of the p38MAPK pathway and contributes to inhibition of angiogenesis. Overexpression in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis. Isoform 3. Mediates the activity of CXCL11.
Alternative names
CD183, GPR9, CXCR3, C-X-C chemokine receptor type 3, CXC-R3, CXCR-3, CKR-L2, G protein-coupled receptor 9, Interferon-inducible protein 10 receptor, IP-10 receptor
Recommended products
Rabbit Recombinant Monoclonal CXCR3 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23845-44
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab274420 is the carrier-free version of Anti-CXCR3 antibody [EPR23845-44] ab259865.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The chemokine receptor CXCR3 also known as CD183 is a protein that plays an important role in immune responses. This receptor is a part of the G protein-coupled receptor (GPCR) family and weighs approximately 41 kDa. CXCR3 is expressed on activated T cells some B cells natural killer (NK) cells and certain subsets of dendritic cells. Its expression is mostly found in inflamed tissues and in conditions where the immune system is active such as infections or autoimmune diseases.
- Biological function summary
CXCR3 is involved in directing the migration of immune cells to sites of inflammation. This receptor binds to specific chemokines—CXCL9 CXCL10 and CXCL11—that are produced in response to cytokines like IFN-gamma. CXCR3 does not function as part of a larger protein complex; instead it operates independently facilitating the trafficking and activation of its expressing cells. Its activity is pivotal in immune responses assisting in rapid mobilization and targeting of effector cells to needed areas.
- Pathways
CXCR3 is a component of the chemokine signaling pathway which is important for leukocyte chemotaxis. Within this pathway CXCR3 interacts with other receptors and proteins that modulate immune responses such as CXCR4 and CCR5 which also mediate chemotactic migration of leukocytes. Moreover it plays a role in the Jak-STAT signaling pathway impacting gene transcription and cell survival particularly in response to inflammatory signals.
- Associated diseases and disorders
CXCR3 is closely associated with autoimmune diseases like multiple sclerosis and rheumatoid arthritis due to its role in inflammation and immune cell recruitment. The receptor is also involved in cancer where its dysregulation can impact tumor growth and metastasis. CXCR3 through its interaction with chemokines like CXCL10 can influence the tumor microenvironment by affecting the infiltration and function of immune cells. Understanding these interactions can provide insights into therapeutic strategies targeting CXCR3 in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free (ab274420), expandable thumbnail](https://content.abcam.com/products/images/cxcr3-antibody-epr23845-44-bsa-and-azide-free-ab274420--flow-cytometry-img126350.jpg "Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free (ab274420)")
Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free (ab274420)
This data was developed using Anti-CXCR3 antibody [EPR23845-44] ab259865, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) (treated with 200U/ml Interleukin-2 (IL-2) and 1μg/ml Phytohemagglutinin (PHA) for 6 days) labelling CXCR3 with Anti-CXCR3 antibody [EPR23845-44] ab259865 at 1/500 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or Anti-CXCR3 antibody [EPR23845-44] ab259865. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.
Gated on viable cells.
![Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free (ab274420), expandable thumbnail](https://content.abcam.com/products/images/cxcr3-antibody-epr23845-44-bsa-and-azide-free-ab274420--flow-cytometry-img439607.jpg "Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free (ab274420)")
Flow Cytometry - Anti-CXCR3 antibody [EPR23845-44] - BSA and Azide free (ab274420)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CXCR3 antibody [EPR23845-44] ab259865).
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) (top) or PBMCs treated with 5 µg/ml phytohaemagglutinin (PHA) for 48 hours (bottom), with Anti-CXCR3 antibody [EPR23845-44] ab259865 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 mins on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody Anti-CXCR3 antibody [EPR23845-44] ab259865 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 µl at 1.0 μg/ml (1/2,000 dilution)) for 30 mins on ice. The cells were simultaneously stained with CD4.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 dilutionfor 30 mins on ice
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.
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