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AB324731

Anti-CXCR3 antibody [EPR27377-504]

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Rabbit Recombinant Monoclonal CXCR3 antibody. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD183, Cmkar3, C-X-C chemokine receptor type 3, CXC-R3, CXCR-3, Interferon-inducible protein 10 receptor, IP-10 receptor

4 Images
Flow Cytometry - Anti-CXCR3 antibody [EPR27377-504] (AB324731)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CXCR3 antibody [EPR27377-504] (AB324731)

Flow cytometric analysis of Mouse spleen cell cells labelling CXCR3 with ab324731 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD45R/B220 conjugated to Brilliant Violet 421.

Flow Cytometry - Anti-CXCR3 antibody [EPR27377-504] (AB324731)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CXCR3 antibody [EPR27377-504] (AB324731)

Flow cytometric analysis of Mouse spleen cell cells labelling CXCR3 with ab324731 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD14 conjugated to Brilliant Violet 421.

Flow Cytometry - Anti-CXCR3 antibody [EPR27377-504] (AB324731)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CXCR3 antibody [EPR27377-504] (AB324731)

Flow cytometric analysis of Mouse spleen cell cells labelling CXCR3 with ab324731 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD4 conjugated to Alexa Fluor®647.
PMID : 11290763, 9116188.

Flow Cytometry - Anti-CXCR3 antibody [EPR27377-504] (AB324731)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CXCR3 antibody [EPR27377-504] (AB324731)

Flow cytometric analysis of Mouse spleen cell cells labelling CXCR3 with ab324731 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD8 conjugated to Alexa Fluor®647.
PMID : 11290763, 9116188.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27377-504

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of mesangial cells through a heterotrimeric G-protein signaling pathway. Probably promotes cell chemotaxis response (By similarity). Binds to CCL21. Upon activation by PF4, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling.
See full target information Cxcr3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com