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Rabbit Recombinant Multiclonal CXCR4 phospho S324 + S325 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CXCR4 phospho S324 + S325.

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Images

Western blot - Anti-CXCR4 (phospho S324 + S325) Antibody [RP23040164] (AB313465), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 (phospho S324 + S325) Antibody [RP23040164] (AB313465), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Synthetic Peptide within Human CXCR4 phospho S324 + S325. Database link P61073

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
2.5 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Target data

Function

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation (PubMed:10452968, PubMed:18799424, PubMed:24912431, PubMed:28978524). Involved in the AKT signaling cascade (PubMed:24912431). Plays a role in regulation of cell migration, e.g. during wound healing (PubMed:28978524). Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels (PubMed:20228059). Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205). Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival (By similarity). (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) for human immunodeficiency virus-1/HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus (PubMed:10074122, PubMed:10756055, PubMed:8849450, PubMed:8929542, PubMed:9427609).

Alternative names

CD184, C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, LESTR, LAP-3, LPS-associated protein 3, SDF-1 receptor, CXCR4

Recommended products

Rabbit Recombinant Multiclonal CXCR4 phospho S324 + S325 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CXCR4 phospho S324 + S325.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Synthetic Peptide within Human CXCR4 phospho S324 + S325. Database link P61073
Clone number
RP23040164
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CXCR4 also known as C-X-C chemokine receptor type 4 is a G protein-coupled receptor that is involved in signal transduction. It has a molecular weight of approximately 41 kDa. CXCR4 is ubiquitously expressed across various tissues including immune cells like T and B lymphocytes as well as in bone marrow brain and heart. It binds specifically with the ligand CXCL12 also known as stromal cell-derived factor 1 (SDF-1) facilitating responses such as cell migration and proliferation.

Biological function summary

CXCR4 plays an important role in the immune system hematopoiesis and angiogenesis. It does not function alone and is often part of a larger protein complex where it recruits and activates other G proteins. The receptor mediates chemotactic responses directing cells to sites of inflammation or injury. Its interaction with CXCL12 is critical for maintaining immune surveillance aiding in the movement and positioning of immune cells.

Pathways

CXCR4 integrates into significant cellular signaling pathways such as the PI3K/AKT pathway and the MAPK pathway. It collaborates closely with signaling proteins like AKT1 and MAPK1 impacting cell survival and growth. These pathways are essential for various cellular functions including cell cycle progression and apoptosis regulation. The cross-talk between CXCR4 and these pathways underlines its influence on cell fate decisions.

Associated diseases and disorders

CXCR4 is implicated in cancer metastasis and HIV entry into cells. Overexpression of CXCR4 is observed in several cancers contributing to tumor growth and metastasis. The interaction between CXCR4 and CXCL12 facilitates the infiltration and spread of cancer cells. Additionally in HIV CXCR4 serves as a coreceptor along with CD4 allowing the virus to enter and infect host cells. Both cancer and HIV illustrate CXCR4's central role in disease progression and pathogenesis.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Western blot - Anti-CXCR4 (phospho S324 + S325) Antibody [RP23040164] (ab313465), expandable thumbnail

    Western blot - Anti-CXCR4 (phospho S324 + S325) Antibody [RP23040164] (ab313465)

    A 55 kDa band corresponding to CXCR4 [pS324/425] was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using 4-12% Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk.

    All lanes: Western blot - Anti-CXCR4 (phospho S324 + S325) Antibody [RP23040164] (ab313465) at 2.5 µg/mL

    Lane 1: Membrane enriched extracts of HEK-293 at 30 µg

    Lane 2: Membrane enriched extracts of A549 at 30 µg

    Lane 3: Membrane enriched extracts of MCF-7 at 30 µg

    Lane 4: Membrane enriched extracts of LNCaP at 30 µg

    Lane 5: Membrane enriched extracts of HeLa at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

    Developed using the ECL technique.

  • Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 (phospho S324 + S325) Antibody [RP23040164] (ab313465), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 (phospho S324 + S325) Antibody [RP23040164] (ab313465)

    For immunofluorescence analysis of Jurkat cells fixed and permeabilized for detection of endogenous CXCR4 [pS324/325] using ab313465 (5 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate ( 1:2000). Nuclei (blue) were stained using with DAPI , and Rhodamine Phalloidin ( 1:300) was used for cytoskeletal F-actin (red) staining. Detection and localization of CXCR4 [pS324/325] (green)on the membrane can be clearly observed in cells treated with PMA (40 ug/ml, 30 min) as compared to untreated cells. Antibody specificity was demonstrated by competition with the CXCR4 [pS324/325] phospho peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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