AB74012

Anti-CXCR4 (phospho S339) antibody

Name: Anti-CXCR4 (phospho S339) antibody (ab74012) | Abcam
Brand: Abcam Limited
SKU: AB74012
Price: 585 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(28 Publications)

Rabbit Polyclonal CXCR4 phospho S339 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human CXCR4 phospho S339.

View Alternative Names

CD184, C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, LESTR, LAP-3, LPS-associated protein 3, SDF-1 receptor, CXCR4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 (phospho S339) antibody (AB74012)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 (phospho S339) antibody (AB74012)

Immunofluorescence analysis of HeLa cells using ab74012, at 1/500 dilution, in the presence (right panel) or absence (left panel) of the immunising phosphopeptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR4 (phospho S339) antibody (AB74012)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR4 (phospho S339) antibody (AB74012)

Immunohistochemistry analysis of paraffin-embedded human brain carcinoma tissue using ab74012, at 1/50 dilution, in the presence (right panel) or absence (left panel) of the immunising phosphopeptide.

Unknown

Western blot - Anti-CXCR4 (phospho S339) antibody (AB74012)

All lanes:

Western blot - Anti-CXCR4 (phospho S339) antibody (ab74012) at 1/500 dilution

Lane 1:

Extracts from HUVEC cells treated with etoposide (25uM, 24hours) at 5 µg

Lane 2:

Extracts from HUVEC cells treated with etoposide (25uM, 24hours) at 5 µg with the immunising phosphopeptide

Predicted band size: 39 kDa

Observed band size: 45 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

ELISA, ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CXCR4 phospho S339. The exact immunogen used to generate this antibody is proprietary information.

P61073

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab74012 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCR4 also known as C-X-C chemokine receptor type 4 is a G protein-coupled receptor that is involved in signal transduction. It has a molecular weight of approximately 41 kDa. CXCR4 is ubiquitously expressed across various tissues including immune cells like T and B lymphocytes as well as in bone marrow brain and heart. It binds specifically with the ligand CXCL12 also known as stromal cell-derived factor 1 (SDF-1) facilitating responses such as cell migration and proliferation.

Biological function summary

CXCR4 plays an important role in the immune system hematopoiesis and angiogenesis. It does not function alone and is often part of a larger protein complex where it recruits and activates other G proteins. The receptor mediates chemotactic responses directing cells to sites of inflammation or injury. Its interaction with CXCL12 is critical for maintaining immune surveillance aiding in the movement and positioning of immune cells.

Pathways

CXCR4 integrates into significant cellular signaling pathways such as the PI3K/AKT pathway and the MAPK pathway. It collaborates closely with signaling proteins like AKT1 and MAPK1 impacting cell survival and growth. These pathways are essential for various cellular functions including cell cycle progression and apoptosis regulation. The cross-talk between CXCR4 and these pathways underlines its influence on cell fate decisions.

CXCR4 is implicated in cancer metastasis and HIV entry into cells. Overexpression of CXCR4 is observed in several cancers contributing to tumor growth and metastasis. The interaction between CXCR4 and CXCL12 facilitates the infiltration and spread of cancer cells. Additionally in HIV CXCR4 serves as a coreceptor along with CD4 allowing the virus to enter and infect host cells. Both cancer and HIV illustrate CXCR4's central role in disease progression and pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation (PubMed : 10452968, PubMed : 18799424, PubMed : 24912431, PubMed : 28978524). Involved in the AKT signaling cascade (PubMed : 24912431). Plays a role in regulation of cell migration, e.g. during wound healing (PubMed : 28978524). Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels (PubMed : 20228059). Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed : 11276205). Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival (By similarity).. (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) for human immunodeficiency virus-1/HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus (PubMed : 10074122, PubMed : 10756055, PubMed : 8849450, PubMed : 8929542, PubMed : 9427609).

See full target information CXCR4 phospho S339

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 26:2471631 PubMed39992057

2025

CXCR4 confers stemness and radioresistance in chordoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chan-Woong Jung,Jeong-Yub Kim,Myung-Jin Park

Cancers 15: PubMed37444550

2023

Co-Expression of Immunohistochemical Markers MRP2, CXCR4, and PD-L1 in Gallbladder Tumors Is Associated with Prolonged Patient Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés Tittarelli,Omar Barría,Evy Sanders,Anna Bergqvist,Daniel Uribe Brange,Mabel Vidal,María Alejandra Gleisner,Jorge Ramón Vergara,Ignacio Niechi,Iván Flores,Cristián Pereda,Cristian Carrasco,Claudia Quezada-Monrás,Flavio Salazar-Onfray

BMC endocrine disorders 22:292 PubMed36419107

2022

The prognostic significance of CXCR4 and SDF-1 in differentiated thyroid cancer depends on CD8+ density.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Wilhelm,Isabelle Lemmenmeier,Alexandros Lalos,Alberto Posabella,Venkatesh Kancherla,Salvatore Piscuoglio,Tarik Delko,Markus von Flüe,Kathrin Glatz,Raoul André Droeser

BMC cancer 22:376 PubMed35397601

2022

High ratio of pCXCR4/CXCR4 tumor infiltrating immune cells in primary high grade ovarian cancer is indicative for response to chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Fabio Walther,Jana Ladina Berther,Alexandros Lalos,Michaela Ramser,Simone Eichelberger,Robert Mechera,Savas Soysal,Simone Muenst,Alberto Posabella,Uwe Güth,Sylvia Stadlmann,Luigi Terracciano,Raoul A Droeser,Jasmin Zeindler,Gad Singer

Molecular neurobiology 58:5421-5436 PubMed33983546

2021

Methamphetamine Enhances HIV-Induced Aberrant Proliferation of Neural Progenitor Cells via the FOXO3-Mediated Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Minseon Park,William Baker,Dilraj Cambow,Danielle Gogerty,Ana Rachel Leda,Bridget Herlihy,Darya Pavlenko,Schuyler Van Den Nieuwenhuizen,Michal Toborek

BioMed research international 2021:8883800 PubMed33829065

2021

Determination of Potential Therapeutic Targets and Prognostic Markers of Ovarian Cancer by Bioinformatics Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Shouguo Huang,Lini Quan,Qiu Meng,Haiyan Wang,Jie Wang,Jin Chen

American journal of translational research 12:2570-2584 PubMed32655791

2020

The CXCR4/miR-125b/FoxP3 axis regulates the function of the epithelial barrier via autophagy in allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zheng,Min Zeng,Jia-Bin Nian,Lian-Ya Zeng,Zhi Fu,Qiu-Ju Huang,Xin Wei

American journal of physiology. Heart and circulatory physiology 318:H1420-H1435 PubMed32330088

2020

TLR2/CXCR4 coassociation facilitates infection-induced atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Guolin Miao,Xi Zhao,Beibei Wang,Lijun Zhang,Guangyan Wang,Ningbo Zheng,Jingya Liu,Zhelong Xu,Lijun Zhang

OncoTargets and therapy 13:2899-2910 PubMed32308422

2020

Long Non-Coding RNA FEZF1-AS1 Modulates CXCR4 to Promote Cell Proliferation, Warburg Effect and Suppress Cell Apoptosis in Osteosarcoma by Sponging miR-144.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liu,Guang Feng,Zhengwei Li,Rui Li,Peng Xia

Carcinogenesis 41:817-827 PubMed31430364

2019

Truncation of MYH8 tail in AML: a novel prognostic marker with increase cell migration and epithelial-mesenchymal transition utilizing RAF/MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hyejoo Park,Daeyoon Kim,Dongchan Kim,Jihyun Park,Youngil Koh,Sung-Soo Yoon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCR4 (phospho S339) antibody

Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 (phospho S339) antibody (AB74012)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR4 (phospho S339) antibody (AB74012)

Western blot - Anti-CXCR4 (phospho S339) antibody (AB74012)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (28)

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