Rabbit Recombinant Monoclonal CXCR5 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt, WB and reacts with Human, Rat, Mouse samples.
Images
![Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (AB272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--flow-cytometry-img13375.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (AB272936)")
![Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (AB272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--flow-cytometry-img118995.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (AB272936)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (AB272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107042.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (AB272936)")
![Western blot - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (AB272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--western-blot-img12397.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (AB272936)")
![Immunocytochemistry/ Immunofluorescence - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (AB272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--immunocytochemistry-immunofluorescence-img65892.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (AB272936)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Expected | Not recommended | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes We observe only weak staining in human WB. |
Species Rat | Dilution info - | Notes We observe only weak staining in human WB. |
Species Human | Dilution info - | Notes We observe only weak staining in human WB. |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
Alternative names
CD185, BLR1, MDR15, CXCR5, C-X-C chemokine receptor type 5, CXC-R5, CXCR-5, Burkitt lymphoma receptor 1, Monocyte-derived receptor 15, MDR-15
Recommended products
Rabbit Recombinant Monoclonal CXCR5 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt, WB and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23463-30
- Purification technique
- Affinity purification Protein A
- Specificity
We observe only weak staining in human WB. We do not suggest this product for use in IHC with mouse or rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab272936 is the carrier-free version of Anti-CXCR5 antibody [EPR23463-30] ab254415.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CXCR5 also known as CD185 is a chemokine receptor with a mass of approximately 43 kDa. It is part of the G protein-coupled receptor family and is widely expressed on follicular B helper T (Tfh) cells B cells and a subset of circulating blood cells. CXCR5 is recognized as a marker for Tfh cells which are critical for the function and maturation of B cells within germinal centers. In tissues CXCR5 expression appears prominently in regions where immune cell interactions facilitate immune and inflammatory responses.
- Biological function summary
CXCR5 serves a significant role in directing B cells and Tfh cells to lymphoid tissues by interacting with its ligand CXCL13. This interaction involves recruitment and organization of B cells into follicles within secondary lymphoid organs. The presence of CXCR5 allows B cells and Tfh cells to navigate the follicular zones efficiently enhance antigen-specific immune responses and contribute to long-lived humoral immunity. CXCR5 functions as part of a complex that includes signaling pathways influencing cellular movements and location.
- Pathways
CXCR5 plays a significant part in the immune system's trafficking pathways particularly within the crosstalk between chemokines and their receptors. CXCR5 expression facilitates the CXC chemokine pathway which important for positioning immune cells within lymphoid organs. Additionally CXCR5 interacts with proteins such as CXCL13 working together to pattern the architecture of germinal centers where affinity maturation and class switching of antibodies occur critical processes in adaptive immunity.
- Associated diseases and disorders
CXCR5 has been implicated in autoimmune diseases and certain lymphomas. For example aberrant CXCR5 expression is linked to the pathogenesis of diseases like systemic lupus erythematosus (SLE) where it might contribute to immune dysregulation and chronic inflammation. In the context of certain lymphomas CXCR5's role in lymphoid organ architecture and cell migration becomes even more relevant. Its expression often parallels the presence of CXCL13 hinting at a network of interactions promoting tumor survival and growth within lymphoid landscapes. Understanding these relationships can offer insight into developing therapeutic strategies potentially involving CXCR5 inhibitors or anti-CXCR5 antibodies to address these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--flow-cytometry-img13375.jpg "Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CXCR5 antibody [EPR23463-30] ab254415)
Flow cytometry overlay histogram showing wild-type Raji (green line) and CXCR5 knockout Raji cells (Human CXCR5 knockout Raji cell line ab273380) stained with Anti-CXCR5 antibody [EPR23463-30] ab254415 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serumto block FC receptors and non-specific protein-protein interaction followed by the antibody (Anti-CXCR5 antibody [EPR23463-30] ab254415) (1x106 in 100μl at 0.2 μg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was Rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) used at the same concentration and conditions as the primary antibody (wild-type Raji - black line; CXCR5 knockout Raji - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
![Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--flow-cytometry-img118995.jpg "Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CXCR5 with Anti-CXCR5 antibody [EPR23463-30] ab254415 at 1/500 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary at a 1/2000 dilution. Cells were stained with rabbit IgG (Left) or Anti-CXCR5 antibody [EPR23463-30] ab254415 (Right), then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CXCR5 antibody [EPR23463-30] ab254415).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107042.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CXCR5 with Anti-CXCR5 antibody [EPR23463-30] ab254415 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human tonsil (PMID: 12393412). The section was incubated with Anti-CXCR5 antibody [EPR23463-30] ab254415 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CXCR5 antibody [EPR23463-30] ab254415).
![Western blot - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--western-blot-img12397.jpg "Western blot - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
Western blot - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CXCR5 antibody [EPR23463-30] ab254415).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-CXCR5 antibody [EPR23463-30] ab254415 observed at 60 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
Anti-CXCR5 antibody [EPR23463-30] ab254415 was shown to react with CXCR5 in Raji wild-type cells in Western blot with loss of signal observed in CXCR5 knockout sample. Wild-type and CXCR5 knockout Raji cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with Anti-CXCR5 antibody [EPR23463-30] ab254415 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-CXCR5 antibody [EPR23463-30] (Anti-CXCR5 antibody [EPR23463-30] ab254415) at 1/1000 dilution
Lane 1: Wild-type Raji cell lysate at 30 µg
Lane 2: CXCR5 knockout Raji cell lysate at 30 µg
Lane 2: Western blot - Human CXCR5 knockout Raji cell line (Human CXCR5 knockout Raji cell line ab273380)
Lane 3: Daudi cell lysate at 30 µg
Lane 4: Jurkat cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 42 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--immunocytochemistry-immunofluorescence-img65892.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
Immunocytochemistry/ Immunofluorescence - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CXCR5 antibody [EPR23463-30] ab254415). Anti-CXCR5 antibody [EPR23463-30] ab254415 staining CXCR5 in Daudi cells (top panel, positive control) and Jurkat cells (bottom panel, negative control). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-CXCR5 antibody [EPR23463-30] ab254415 at 5μg/ml concentration and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107528.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling CXCR5 with Anti-CXCR5 antibody [EPR23463-30] ab254415 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human diffuse large B-cell lymphoma. The section was incubated with Anti-CXCR5 antibody [EPR23463-30] ab254415 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CXCR5 antibody [EPR23463-30] ab254415).
![Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--flow-cytometry-img414214.jpg "Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)
Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling CXCR5 with Anti-CXCR5 antibody [EPR23463-30] ab254415 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CXCR5 antibody [EPR23463-30] ab254415).
![Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--flow-cytometry-img439649.jpg "Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CXCR5 antibody [EPR23463-30] ab254415).
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with Anti-CXCR5 antibody [EPR23463-30] ab254415 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left). PBMCs were incubated for 30 mins on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody Anti-CXCR5 antibody [EPR23463-30] ab254415 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (1x 106 in 100 µl at 0.2 μg/ml (1/11500 dilution)) for 30 mins on ice. The cells were simultaneously stained with CD19.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 dilution for 30 mins on ice
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107528.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
![Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--flow-cytometry-img414214.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
![Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936), expandable thumbnail](https://content.abcam.com/products/images/cxcr5-antibody-epr23463-30-bsa-and-azide-free-ab272936--flow-cytometry-img439649.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (ab272936)")
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