Anti-CXCR5 antibody [HL3708] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (AB325850)

ab325850 detects CXCR5 protein by immunohistochemical analysis. Sample : Paraffin-embedded human tissues. CXCR5 stained by ab325850 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (AB325850)

ab325850 detects CXCR5 protein by immunohistochemical analysis. Sample : Paraffin-embedded human tonsil. CXCR5 stained by ab325850 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (AB325850)

ab325850 detects CXCR5 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. ab325850 diluted at 1 : 250. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (AB325850)

ab325850 detects CXCR5 protein by immunohistochemical analysis. Sample : Frozen-sectioned mouse colon. Green : CXCR5 stained by ab325850 diluted at 1 : 250. Red : Vimentin antibody diluted at 1 : 1000. Blue : Hoechst 33342 staining.

• WB

Supplier Data

Western blot - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (AB325850)

Unboiled various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325850 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (ab325850) at 1/1000 dilution

Lane 1:

Unboiled Daudi whole cell extracts at 30 µg

Lane 2:

Unboiled Jurkat whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (AB325850)

Untreated (–) and treated (+) unboiled Raji whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325850 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (ab325850) at 1/1000 dilution

Lane 1:

Untreated (–) unboiled Raji whole cell extracts at 30 µg

Lane 2:

PNGase F, 2 hr treated (+) unboiled Raji whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (AB325850)

Boiled and unboiled rat tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325850 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (ab325850) at 1/1000 dilution

Lane 1:

Boiled rat spleen tissue extracts at 50 µg

Lane 2:

Unboiled rat spleen tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (AB325850)

Boiled and unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325850 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (ab325850) at 1/1000 dilution

Lane 1:

Boiled mouse spleen tissue extracts at 50 µg

Lane 2:

Boiled mouse brain tissue extracts at 50 µg

Lane 3:

Unboiled mouse spleen tissue extracts at 50 µg

Lane 4:

Unboiled mouse brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (AB325850)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325850 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CXCR5 antibody [HL3708] - BSA and Azide free (ab325850) at 1/5000 dilution

Lanes 1, 3, 5, 7, 9, 11, 13 and 15:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled CXCR1 transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled CXCR2 transfected (+) 293T whole cell extracts at 30 µg

Lane 6:

Unboiled CXCR4 transfected (+) 293T whole cell extracts at 30 µg

Lane 8:

Unboiled CXCR5 transfected (+) 293T whole cell extracts at 30 µg

Lane 10:

Unboiled CXCR6 transfected (+) 293T whole cell extracts at 30 µg

Lane 12:

Unboiled CXCR7 transfected (+) 293T whole cell extracts at 30 µg

Lane 14:

Unboiled CX3CR1 transfected (+) 293T whole cell extracts at 30 µg

Lane 16:

Unboiled ACKR4 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false