AB281934

Anti-CXCR6 antibody [EPR24686-113] - BSA and Azide free

Name: Anti-CXCR6 antibody [EPR24686-113] - BSA and Azide free (ab281934) | Abcam
Brand: Abcam Limited
SKU: AB281934
Price: 905 USD
Availability: InStock
Rating: 2 (3 reviews)

BOND RX™ Validated
RabMAb
Recombinant
What is this?

(3 Reviews)

(0 Publication)

Rabbit Recombinant Monoclonal CXCR6 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

CD186, BONZO, STRL33, TYMSTR, CXCR6, C-X-C chemokine receptor type 6, CXC-R6, CXCR-6, CDw186, G-protein coupled receptor STRL33, G-protein coupled receptor bonzo

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] - BSA and Azide free (AB281934)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] - BSA and Azide free (AB281934)

This data was developed using ab273116, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling CXCR6 with ab273116 at 1/1000 dilution (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in human cerebrum (PMID : 9230441). The section was incubated with ab273116 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] - BSA and Azide free (AB281934)

This data was developed using ab273116, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human diffuse large B cell lymphoma tissue labelling CXCR6 with ab273116 at 1/1000 dilution (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous and cytoplasmic staining in human diffuse large B cell lymphoma. The section was incubated with ab273116 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] - BSA and Azide free (AB281934)

This data was developed using ab273116, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CXCR6 with ab273116 at 1/1000 dilution (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous and cytoplasmic staining on scattered immune cells of human tonsil. The section was incubated with ab273116 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Unconjugated

Anti-CXCR6 antibody [EPR24686-113]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24686-113

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab281934 is the carrier-free version of ab273116.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCR6 also known as CD186 or BONZO is a chemokine receptor that belongs to the larger family of G protein-coupled receptors. This receptor has a molecular weight of approximately 45 kDa. CXCR6 gets expressed on various leukocytes including T-cells and natural killer cells. This expression profiles CXCR6 as essential in the immune system where its occupation involves directing cell movement via chemotaxis a process integral for normal immune surveillance and inflammatory response.

Biological function summary

CXCR6 critically orchestrates the interactions between T-cells and antigen-presenting cells within tissues. By engaging its ligand CXCL16 CXCR6 helps mediate cell adhesion and migration. This receptor works as part of an immune complex influencing the migration and retention of immune cells at sites of inflammation. The presence of CXCR6 enhances tissue-specific homing which is vital for localized immune responses.

Pathways

CXCR6 plays a significant role in the chemokine signaling pathway. This pathway leads immune cells to sites of inflammation or infection. Moreover CXCR6 interacts closely with other chemokine receptors such as CCR5. These receptor interactions ensure efficient communication and coordination in cell trafficking across immune environments all of which are critical for proper immune function.

CXCR6 has a known association with diseases like HIV and certain forms of cancer. CXCR6 can act as a co-receptor for the entry of some HIV strains into cells linking it to viral pathogenesis. In the context of cancer CXCR6 and its ligand CXCL16 have connections with tumor cell migration and metastasis. The interaction of CXCR6 with proteins like CXCL16 creates a microenvironment that can either suppress or promote tumor progression depending on the context.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1.

See full target information CXCR6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCR6 antibody [EPR24686-113] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] - BSA and Azide free (AB281934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] - BSA and Azide free (AB281934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] - BSA and Azide free (AB281934)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise