JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB133191

Anti-CXXC5 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal CXXC5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CXXC5.

View Alternative Names

HSPC195, TCCCIA00297, CXXC5, CXXC-type zinc finger protein 5, CF5, Putative MAPK-activating protein PM08, Putative NF-kappa-B-activating protein 102, Retinoid-inducible nuclear factor, RINF

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXXC5 antibody (AB133191)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXXC5 antibody (AB133191)

ab133191, at 5 µg/ml, staining CXXC5 in Human pancreas tissue by immunohistochemistry [Formalin fixed and Paraffin Embedded Tissues (IHC-P)] followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Western blot - Anti-CXXC5 antibody (AB133191)
  • WB

Supplier Data

Western blot - Anti-CXXC5 antibody (AB133191)

All lanes:

Western blot - Anti-CXXC5 antibody (ab133191) at 1 µg/mL

All lanes:

Human brain tissue lysate

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CXXC5. The exact immunogen used to generate this antibody is proprietary information.

Q7LFL8

Specificity

Human CXXC5. At least two isoforms of CXXC5 are known to exist. CXXC5 antibody is predicted to not cross-react with other CXXC protein family members.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXXC5 also known as CXXC finger protein 5 is a DNA-binding protein with a molecular weight of approximately 35 kDa. This protein is expressed in various tissues including the brain and bone marrow. It functions mechanically by binding to unmethylated CpG dinucleotides influencing gene expression by affecting chromatin structure. Due to its DNA-binding ability CXXC5 can regulate transcription impacting various cellular processes.
Biological function summary

CXXC5 plays a role in the regulation of Wnt/β-catenin signaling pathway and is involved in controlling cellular proliferation and differentiation. It does not form part of a larger protein complex but it interacts with other proteins to exert its function. CXXC5 can act as a transcriptional regulator by recruiting other proteins to modify epigenetic marks and modulates pathways critical for development and disease.

Pathways

CXXC5 is involved in the Wnt/β-catenin signaling cascade a major pathway that regulates cell fate decisions migration and organogenesis. This protein bears relation to other pathway components such as β-catenin by interacting with them to convey signaling outcomes during cellular processes. In the bone it influences osteoblastic differentiation and bone formation making it an important component in skeletal development.

CXXC5 has been linked to conditions such as osteoporosis and certain types of cancer. In osteoporosis the altered function of CXXC5 affects bone density due to its role in bone anabolic responses with interactions with β-catenin being important. In cancer disruptions in CXXC5 expression or function can lead to uncontrolled cell proliferation and it is often examined in relation to tumor suppressor pathways highlighting its role in cellular growth control mechanisms.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May indirectly participate in activation of the NF-kappa-B and MAPK pathways. Acts as a mediator of BMP4-mediated modulation of canonical Wnt signaling activity in neural stem cells (By similarity). Required for DNA damage-induced ATM phosphorylation, p53 activation and cell cycle arrest. Involved in myelopoiesis. Transcription factor. Binds to the oxygen responsive element of COX4I2 and represses its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen) (PubMed : 23303788). May repress COX4I2 transactivation induced by CHCHD2 and RBPJ (PubMed : 23303788). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed : 29276034).
See full target information CXXC5
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of experimental pathology 98:234-243 PubMed29027288

2017

CXXC5 expression in prostate cancer: implications for cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ines Benedetti,Angelo M De Marzo,Jan Geliebter,Niradiz Reyes

Proceedings of the National Academy of Sciences of 113:12478-12483 PubMed27738243

2016

Autotaxin-mediated lipid signaling intersects with LIF and BMP signaling to promote the naive pluripotency transcription factor program.

Applications

Unspecified application

Species

Unspecified reactive species

Cody Kime,Masayo Sakaki-Yumoto,Leeanne Goodrich,Yohei Hayashi,Salma Sami,Rik Derynck,Michio Asahi,Barbara Panning,Shinya Yamanaka,Kiichiro Tomoda
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB106533

Anti-CXXC5 antibody

Western blot - Anti-CXXC5 antibody (AB106533)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB235358

Anti-ASF1b antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASF1b antibody (AB235358)

  • 0
  • 1
Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com