AB11309

Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker

Name: Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker (ab11309) | Abcam
Brand: Abcam Limited
SKU: AB11309
Price: 545 USD
Availability: InStock
Rating: 5 (11 reviews)

(11 Reviews)

(12 Publications)

Mouse Monoclonal TBB2 antibody - conjugated to Cy3®. Suitable for ICC/IF and reacts with Chicken samples. Cited in 12 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Tubb5.

View Alternative Names

Tubulin beta-2 chain, Beta-tubulin class-II

1 Images

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker (AB11309)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker (AB11309)

Immunofluorescent analysis of methanol fixed, acetone permeabilized chicken fibroblast cells labelling beta-Tubulin-Cy3 using ab11309 at 1/200 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TUB 2.1

Isotype

IgG1

Conjugation

Cy3®

Excitation/Emission

Ex: 552nm, Em: 565nm

Carrier free

Reacts with

Chicken

Applications

ICC/IF

applications

Immunogen

Full Length Protein corresponding to Rat Tubb5. The exact immunogen used to generate this antibody is proprietary information.

P69897

Epitope

Ab11309 specifically recognizes an epitope in the carboxy-terminal part of beta-tubulin (between amino acids 281-446).

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chicken": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

Product details

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

The product is prepared by conjugation of Cy3 to purified monoclonal anti-beta-tubulin antibody. The conjugate is purified by gel filtration to remove unbound Cy3 fluorophore. F/P Molar Ratio: (Cy3:Ab) 3 to 9.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TBB2_CHICK

style

left-column

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 9:105 PubMed37875488

2023

Ectopic expression of human TUBB8 leads to increased aneuploidy in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Dong,Liping Jin,Shihua Bao,Biaobang Chen,Yang Zeng,Yuxi Luo,Xingzhu Du,Qing Sang,Tianyu Wu,Lei Wang

Frontiers in immunology 13:978824 PubMed36268025

2022

Comprehensive single cell analysis of pandemic influenza A virus infection in the human airways uncovers cell-type specific host transcriptional signatures relevant for disease progression and pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna N Kelly,Laura Laloli,Philip V'kovski,Melle Holwerda,Jasmine Portmann,Volker Thiel,Ronald Dijkman

Frontiers in medicine 9:741989 PubMed35280870

2022

Altered Differentiation and Inflammation Profiles Contribute to Enhanced Innate Responses in Severe COPD Epithelium to Rhinovirus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Guo-Parke,Dermot Linden,Aurelie Mousnier,Ian C Scott,Helen Killick,Lee A Borthwick,Andrew J Fisher,Sinéad Weldon,Clifford C Taggart,Joseph C Kidney

Journal of biomechanics 129:110774 PubMed34627073

2021

Effects of cyclic tensile strain and microgravity on the distribution of actin fiber and Fat1 cadherin in murine articular chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Junpei Hatakeyama,Masato Nomura,Yoshio Wakimoto,Shota Inoue,Changxin Li,Daisuke Takamura,Toshihiro Akisue,Hideki Moriyama

Molecular & cellular proteomics : MCP 19:793-807 PubMed32075873

2020

The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Olivier Touzelet,Lindsay Broadbent,Stuart D Armstrong,Waleed Aljabr,Elaine Cloutman-Green,Ultan F Power,Julian A Hiscox

PLoS genetics 15:e1008261 PubMed31860668

2019

Restricted and non-essential redundancy of RNAi and piRNA pathways in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Eliska Taborska,Josef Pasulka,Radek Malik,Filip Horvat,Irena Jenickova,Zoe Jelić Matošević,Petr Svoboda

Frontiers in microbiology 10:811 PubMed31057517

2019

The Human Upper Respiratory Tract Epithelium Is Susceptible to Flaviviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie J Vielle,Obdulio García-Nicolás,Blandina I Oliveira Esteves,Melanie Brügger,Artur Summerfield,Marco P Alves

The Journal of neuroscience : the official journal 39:2011-2024 PubMed30647150

2019

Knockdown of Fidgetin Improves Regeneration of Injured Axons by a Microtubule-Based Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew J Matamoros,Veronica J Tom,Di Wu,Yash Rao,David J Sharp,Peter W Baas

PloS one 13:e0201328 PubMed30517096

2018

Characterisation of morphological differences in well-differentiated nasal epithelial cell cultures from preterm and term infants at birth and one-year.

Applications

Unspecified application

Species

Unspecified reactive species

Helen E Groves,Hong Guo-Parke,Lindsay Broadbent,Michael D Shields,Ultan F Power

Molecular systems biology 14:e8238 PubMed30104419

2018

Unraveling mitotic protein networks by 3D multiplexed epitope drug screening.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenz J Maier,Stefan M Kallenberger,Katharina Jechow,Marcel Waschow,Roland Eils,Christian Conrad

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-beta Tubulin antibody [TUB 2.1] – Microtubule Marker (AB11309)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise