Rabbit Polyclonal LAMP1 antibody - conjugated to Cy3®. Suitable for ICC/IF and reacts with Mouse, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human LAMP1 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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- Biophysical journal 121:1205-12182022Transport of lysosomes decreases in the perinuclear region: Insights from changepoint analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan T Rayens,Keisha J Cook,Scott A McKinley,Christine K PaynePubMed 35202608
- JCI insight 6:2021Resolving monocytes generated through TRAM deletion attenuate atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuo Geng,Yao Zhang,Ziyue Yi,Ran Lu,Liwu LiPubMed 34499622
- Journal of animal science and biotechnology 12:632021Overexpression of Toll-like receptor 4 contributes to the internalization and elimination of Escherichia coli in sheep by enhancing caveolae-dependent endocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Li,Yue Zhao,Xueling Xu,Rui Zhang,Jinlong Zhang,Xiaosheng Zhang,Yan Li,Shoulong Deng,Zhengxing LianPubMed 33966642
- mSphere 6:2021IL-1R1-Dependent Signals Improve Control of Cytosolic Virulent Mycobacteria .Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanne van der Niet,Maaike van Zon,Karin de Punder,Anita Grootemaat,Sofie Rutten,Simone J C F M Moorlag,Diane Houben,Astrid M van der Sar,Wilbert Bitter,Roland Brosch,Rogelio Hernandez Pando,Maria T Pena,Peter J Peters,Eric A Reits,Katrin D Mayer-Barber,Nicole N van der WelPubMed 33952660
- Science advances 5:eaav23092019Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuo Geng et. al.PubMed 30775441
- FASEB journal : official publication of the Federa 31:4140-41522017Interleukin 6 protects pancreatic β cells from apoptosis by stimulation of autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmelia K Linnemann et. al.PubMed 28592636
- Journal of virology 90:8720-82016An Ebola Virus-Like Particle-Based Reporter System Enables Evaluation of Antiviral Drugs In Vivo under Non-Biosafety Level 4 Conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDapeng Li et. al.PubMed 27440895
- Human vaccines & immunotherapeutics 12:77-842015Immuno-potentiating pathway of HBsAg-HBIG immunogenic complex visualized.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHu Liu,Shuang Geng,Bo Wang,Bing Wu,Xiaoping Xie,Shuang Wang,Yiwei Zhong,Xuanyi Wang,Di Qu,Yumei Wen,Bin WangPubMed 26618396
- Immunology and cell biology 93:158-662014Tubulin- and actin-associating GIMAP4 is required for IFN-γ secretion during Th cell differentiation.Applications:IFReactive species:Unspecified reactive speciesMirkka T Heinonen,Kartiek Kanduri,Harri J Lähdesmäki,Riitta Lahesmaa,Tiina A HenttinenPubMed 25287446
- Proceedings of the National Academy of Sciences of 106:8003-82009Alpha5beta1-integrin controls ebolavirus entry by regulating endosomal cathepsins.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Cy3®
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LAMP1 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P11279
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Chicken | Predicted |
| Cow | Predicted |
| Pig | Predicted |
| Woodchuck | Predicted |
| Xenopus laevis | Predicted |
| Zebrafish | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Woodchuck | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed:37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Also plays an important role in NK-cells cytotoxicity (PubMed:2022921, PubMed:23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins (PubMed:7685349). (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:28448640). Promotes also fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909). (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
Alternative names
CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1
Recommended products
Rabbit Polyclonal LAMP1 antibody - conjugated to Cy3®. Suitable for ICC/IF and reacts with Mouse, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human LAMP1 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Cy3®
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LAMP1 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P11279
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Purified using an antigen affinity matrix.The conjugate was purified by gel-filtration to remove unbound Cy3 fluorophore.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Molar Ratio (F/P): 3-9.
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This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.
- Biological function summary
LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.
- Pathways
The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.
- Associated diseases and disorders
LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody (ab67283)
ab67283 staining LAMP1 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.4% saponin. Rabbit Anti-PMP70, Atto 488 Conjugate (peroxisomes stained in green) and 2 μg/mL Rabbit Anti-LAMP1, Cy3 Conjugate (ab67283) (lysosomes stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody (ab67283)
ab67283 (2μg) staining LAMP1 in NIH-3T3 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde.
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