AB67283

Cy3 ® Anti-LAMP1 antibody - Lysosome Marker

Name: Cy3 ® Anti-LAMP1 antibody - Lysosome Marker (ab67283) | Abcam
Brand: Abcam Limited
SKU: AB67283
Price: 430 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(14 Publications)

Rabbit Polyclonal LAMP1 antibody - conjugated to Cy3®. Suitable for ICC/IF and reacts with Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human LAMP1 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1

2 Images

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody - Lysosome Marker (AB67283)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody - Lysosome Marker (AB67283)

ab67283 staining LAMP1 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.4% saponin. Rabbit Anti-PMP70, Atto 488 Conjugate (peroxisomes stained in green) and 2 μg/mL Rabbit Anti-LAMP1, Cy3 Conjugate (ab67283) (lysosomes stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody - Lysosome Marker (AB67283)

ab67283 (2μg) staining LAMP1 in NIH-3T3 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy3®

Excitation/Emission

Ex: 552nm, Em: 565nm

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human LAMP1 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P11279

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Woodchuck": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Molar Ratio (F/P): 3-9.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified using an antigen affinity matrix.The conjugate was purified by gel-filtration to remove unbound Cy3 fluorophore.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed : 37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed : 37390818). Also plays an important role in NK-cells cytotoxicity (PubMed : 2022921, PubMed : 23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed : 23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed : 23847195). Presents carbohydrate ligands to selectins (PubMed : 7685349).. (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed : 24970085, PubMed : 25972533, PubMed : 27605678, PubMed : 28448640). Also promotes fusion of the virus with host membrane in less acidic endosomes (PubMed : 29295909).. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

See full target information LAMP1

style

left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular immunology 21:1522-1534 PubMed39537902

2024

Alternative mRNA polyadenylation regulates macrophage hyperactivation via the autophagy pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzhu Chen,Baiwen Chen,Jingyu Li,Haixin Li,Gaoyang Wang,Xuemin Cai,Qianqian Zhang,Xiaoxu Liu,Chen Kan,Lei Wang,Zhengting Wang,Hua-Bing Li

iScience 27:110097 PubMed38883832

2024

Resolving neutrophils through genetic deletion of TRAM attenuate atherosclerosis pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Geng,Yao Zhang,Ran Lu,Daniel Irimia,Liwu Li

Journal of virology 97:e0028423 PubMed37255472

2023

Critical Role of Viral Protein Hexon in Hypervirulent Fowl Adenovirus Serotype-4-Induced Autophagy by Interaction with BAG3 and Promotion of Viral Replication in LMH Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Guangju You,Areayi Haiyilati,Hongnuan Wang,Huixuan Jiao,Yongqiang Wang,Li Gao,Hong Cao,Xiaoqi Li,Shijun J Zheng

International journal of molecular sciences 23: PubMed36293542

2022

Heparanase Increases Podocyte Survival and Autophagic Flux after Adriamycin-Induced Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hanan Abu-Tayeh Suleiman,Shereen Said,Haya Ali Saleh,Aviva Gamliel-Lazarovich,Eyas Haddad,Irina Minkov,Yaniv Zohar,Neta Ilan,Israel Vlodavsky,Zaid Abassi,Suheir Assady

Biophysical journal 121:1205-1218 PubMed35202608

2022

Transport of lysosomes decreases in the perinuclear region: Insights from changepoint analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan T Rayens,Keisha J Cook,Scott A McKinley,Christine K Payne

JCI insight 6: PubMed34499622

2021

Resolving monocytes generated through TRAM deletion attenuate atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Geng,Yao Zhang,Ziyue Yi,Ran Lu,Liwu Li

Journal of animal science and biotechnology 12:63 PubMed33966642

2021

Overexpression of Toll-like receptor 4 contributes to the internalization and elimination of Escherichia coli in sheep by enhancing caveolae-dependent endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Li,Yue Zhao,Xueling Xu,Rui Zhang,Jinlong Zhang,Xiaosheng Zhang,Yan Li,Shoulong Deng,Zhengxing Lian

mSphere 6: PubMed33952660

2021

IL-1R1-Dependent Signals Improve Control of Cytosolic Virulent Mycobacteria .

Applications

Unspecified application

Species

Unspecified reactive species

Sanne van der Niet,Maaike van Zon,Karin de Punder,Anita Grootemaat,Sofie Rutten,Simone J C F M Moorlag,Diane Houben,Astrid M van der Sar,Wilbert Bitter,Roland Brosch,Rogelio Hernandez Pando,Maria T Pena,Peter J Peters,Eric A Reits,Katrin D Mayer-Barber,Nicole N van der Wel

Science advances 5:eaav2309 PubMed30775441

2019

Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Geng,Yao Zhang,Christina Lee,Liwu Li

FASEB journal : official publication of the Federa 31:4140-4152 PubMed28592636

2017

Interleukin 6 protects pancreatic β cells from apoptosis by stimulation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Amelia K Linnemann,Joseph Blumer,Michelle R Marasco,Therese J Battiola,Heidi M Umhoefer,Jee Young Han,Dudley W Lamming,Dawn Belt Davis

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Cy3 ® Anti-LAMP1 antibody - Lysosome Marker

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody - Lysosome Marker (AB67283)

Immunocytochemistry/ Immunofluorescence - Cy3 ® Anti-LAMP1 antibody - Lysosome Marker (AB67283)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise