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Rat Monoclonal CD19 antibody - conjugated to Cy5®. Suitable for IP, Flow Cyt, FuncS and reacts with Mouse samples. Immunogen corresponding to Cell preparation containing Cd19 protein.

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Images

Flow Cytometry - Cy5 ® Anti-CD19 antibody [6D5] (AB25295), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Rat

Conjugation

Cy5®

Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing Cd19 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P25918

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytFuncS
Mouse
Expected
Tested
Expected

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

ab136584 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Version

8 products for Alternative Product

Target data

Function

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (By similarity). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:20101619, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:7542548, PubMed:7543183, PubMed:9317126). Required for normal differentiation of B-1 cells (PubMed:12387743, PubMed:7542548, PubMed:7543183). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:12387743, PubMed:7542548, PubMed:9317126). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:7542548, PubMed:7543183).

Alternative names

B-lymphocyte antigen CD19, Differentiation antigen CD19, Cd19

Recommended products

Rat Monoclonal CD19 antibody - conjugated to Cy5®. Suitable for IP, Flow Cyt, FuncS and reacts with Mouse samples. Immunogen corresponding to Cell preparation containing Cd19 protein.

Key facts

Isotype

IgG2a

Conjugation

Cy5®

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing Cd19 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P25918
Clone number

6D5

Purification technique

Affinity purification

Light chain type

kappa

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

Associated diseases and disorders

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Flow Cytometry - Cy5 ® Anti-CD19 antibody [6D5] (ab25295), expandable thumbnail

    Flow Cytometry - Cy5 ® Anti-CD19 antibody [6D5] (ab25295)

    Flow cytometric analysis of BALB/c mouse splenocytes labeling CD19 using ab25295 at 0.2 μg/106 cells in 100 μl. CD3 is labeled using a Rat Anti-Mouse CD3 epsilon FITC conjugate.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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