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AB236612

Anti-CYB5B antibody

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(1 Publication)

Rabbit Polyclonal CYB5B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYB5B aa 1 to C-terminus.

View Alternative Names

CYB5M, OMB5, CYB5B, Cytochrome b5 type B, Cytochrome b5 outer mitochondrial membrane isoform

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5B antibody (AB236612)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5B antibody (AB236612)

Paraffin-embedded human kidney tissue stained for CYB5B using ab236612 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CYB5B antibody (AB236612)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CYB5B antibody (AB236612)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CYB5B (green) using ab236612 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-CYB5B antibody (AB236612)
  • WB

Supplier Data

Western blot - Anti-CYB5B antibody (AB236612)

All lanes:

Western blot - Anti-CYB5B antibody (ab236612) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

Mouse liver lysate

Lane 3:

Mouse heart lysate

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 5:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Lane 6:

EC109 whole cell lysate

Lane 7:

A549 (human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CYB5B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43169

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CYB5B protein also known as Cytochrome b5 type B is a microsomal enzyme with an approximate mass of 13.4 kDa. It resides mainly in the endoplasmic reticulum membrane playing a role in electron transfer activities. This protein is expressed in various tissues with notable presence in the liver and adrenal glands. Unlike its mitochondrial counterparts CYB5B is involved in several mixed-function oxidase systems contributing to the catalysis of reactions through electron transfer.
Biological function summary

Cytochrome b5 type B interacts with a wide range of oxidoreductase enzymes which assists in lipid biosynthesis. It acts as a component of the electron transport chain within the endoplasmic reticulum supporting the desaturation and elongation of fatty acids. Though not part of a larger complex its role in electron donation is critical for maintaining cellular lipid homeostasis and synthesizing essential fatty acids.

Pathways

CYB5B plays a role in both the fatty acid metabolic process and the sterol biosynthetic pathway. It provides electrons to desaturase enzymes like stearoyl-CoA desaturase facilitating the transformation of saturated fatty acids into unsaturated fatty acids. This electron relay is also integral to the modulation of cholesterol levels within cells and relates to proteins such as NADH-cytochrome b5 reductase that help transfer electrons from NADH to cytochrome b5.

CYB5B alterations can impact conditions such as fatty liver disease and adrenal hyperplasia. Its functional collaboration with enzymes such as cytochrome P450 isoforms can influence steroid hormone biosynthesis especially in adrenal tissues. The dysregulation of CYB5B activity might affect lipid metabolic stability making it a point of interest in understanding lipid-associated metabolic and endocrine disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.
See full target information CYB5B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 24:534 PubMed38671413

2024

Association of DOK3 and infiltrated tumor-associated macrophages with risk for the prognosis of Porphyromonas gingivalis-infected oral cancer: a 12-year data analysis of 200 patients from a tertiary teaching hospital, Urumqi, China.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Li,Muqiu Li,Wei Wei,Zhengye Wang,Jingwen Yu,Zhongcheng Gong
View all publications
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