AB104217

Anti-CYB5R1 antibody

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(2 Publications)

Rabbit Polyclonal CYB5R1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CYB5R1 aa 150-300.

View Alternative Names

NQO3A2, UNQ3049/PRO9865, CYB5R1, NADH-cytochrome b5 reductase 1, b5R.1, Humb5R2, NAD(P)H:quinone oxidoreductase type 3 polypeptide A2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R1 antibody (AB104217)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R1 antibody (AB104217)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with ab104217 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Unknown

Western blot - Anti-CYB5R1 antibody (AB104217)

10% SDS-PAGE

All lanes:

Western blot - Anti-CYB5R1 antibody (ab104217) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 34 kDa,40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CYB5R1 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UHQ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYB5R1 also known as NADH-cytochrome b5 reductase is an enzyme that plays an important role in electron transfer. It has a molecular mass of approximately 34 kDa and is found in various tissues like liver and erythrocytes. The enzyme exists in different forms within the cell both membrane-bound and soluble which contribute to its diverse functionality. It catalyzes the reduction of cytochrome b5 using NADH as an electron donor acting as a component of the electron transport chain that facilitates different metabolic processes.

Biological function summary

CYB5R1 participates in lipid metabolism and drug metabolism processes. This enzyme works in concert with cytochrome b5 enhancing metabolic activities involving fatty acid desaturation and elongation. It interacts with a wide array of substrates functioning in critical cellular mechanisms including the reduction of methemoglobin to hemoglobin. Although it does not typically form large complexes CYB5R1's activity is essential for maintaining cellular redox balance.

Pathways

CYB5R1 is involved in pathways such as the electron transport chain and fatty acid metabolism. Its activity influences the proper functioning of the electron transfer system by modulating electron transfer to cytochrome b5 which then influences enzymes like fatty acid desaturase. This modulation helps maintain lipid composition within cellular membranes and contributes to energy production in mitochondria.

CYB5R1 has implications in methemoglobinemia and cancer. Mutations or malfunctions in CYB5R1 can lead to an inability to effectively reduce methemoglobin resulting in methemoglobinemia where hemoglobin cannot carry oxygen efficiently. The enzyme's role in drug metabolism also links it to certain cancer types as alterations in its activity can affect drug efficacy and resistance. Its relationships with proteins like cytochrome P450 highlight CYB5R1’s significance in drug metabolism-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

See full target information CYB5R1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e18209 PubMed38682349

2024

Exosomal HSPB1, interacting with FUS protein, suppresses hypoxia-induced ferroptosis in pancreatic cancer by stabilizing Nrf2 mRNA and repressing P450.

Applications

Unspecified application

Species

Unspecified reactive species

Lun Zhang,Liuxu Yang,Keyuan Du

iScience 25:104250 PubMed35521520

2022

Rab33b-exocyst interaction mediates localized secretion for focal adhesion turnover and cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Synne Arstad Bjørnestad,Noemi Antonella Guadagno,Ingrid Kjos,Cinzia Progida

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CYB5R1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R1 antibody (AB104217)

Western blot - Anti-CYB5R1 antibody (AB104217)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise