Rabbit Polyclonal CYB5R1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CYB5R1 aa 1-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CYB5R1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHQ9
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
Alternative names
NQO3A2, UNQ3049/PRO9865, CYB5R1, NADH-cytochrome b5 reductase 1, b5R.1, Humb5R2, NAD(P)H:quinone oxidoreductase type 3 polypeptide A2
Recommended products
Rabbit Polyclonal CYB5R1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CYB5R1 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CYB5R1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHQ9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CYB5R1 also known as NADH-cytochrome b5 reductase is an enzyme that plays an important role in electron transfer. It has a molecular mass of approximately 34 kDa and is found in various tissues like liver and erythrocytes. The enzyme exists in different forms within the cell both membrane-bound and soluble which contribute to its diverse functionality. It catalyzes the reduction of cytochrome b5 using NADH as an electron donor acting as a component of the electron transport chain that facilitates different metabolic processes.
- Biological function summary
CYB5R1 participates in lipid metabolism and drug metabolism processes. This enzyme works in concert with cytochrome b5 enhancing metabolic activities involving fatty acid desaturation and elongation. It interacts with a wide array of substrates functioning in critical cellular mechanisms including the reduction of methemoglobin to hemoglobin. Although it does not typically form large complexes CYB5R1's activity is essential for maintaining cellular redox balance.
- Pathways
CYB5R1 is involved in pathways such as the electron transport chain and fatty acid metabolism. Its activity influences the proper functioning of the electron transfer system by modulating electron transfer to cytochrome b5 which then influences enzymes like fatty acid desaturase. This modulation helps maintain lipid composition within cellular membranes and contributes to energy production in mitochondria.
- Associated diseases and disorders
CYB5R1 has implications in methemoglobinemia and cancer. Mutations or malfunctions in CYB5R1 can lead to an inability to effectively reduce methemoglobin resulting in methemoglobinemia where hemoglobin cannot carry oxygen efficiently. The enzyme's role in drug metabolism also links it to certain cancer types as alterations in its activity can affect drug efficacy and resistance. Its relationships with proteins like cytochrome P450 highlight CYB5R1’s significance in drug metabolism-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-CYB5R1 antibody (ab96103)
10% SDS PAGE
All lanes: Western blot - Anti-CYB5R1 antibody (ab96103) at 1/500 dilution
All lanes: Hela S3 whole cell lysate at 30 µg
Predicted band size: 34 kDa
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