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AB96103

Anti-CYB5R1 antibody

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(1 Publication)

Rabbit Polyclonal CYB5R1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYB5R1 aa 1-250.

View Alternative Names

NQO3A2, UNQ3049/PRO9865, CYB5R1, NADH-cytochrome b5 reductase 1, b5R.1, Humb5R2, NAD(P)H:quinone oxidoreductase type 3 polypeptide A2

1 Images
Western blot - Anti-CYB5R1 antibody (AB96103)
  • WB

Unknown

Western blot - Anti-CYB5R1 antibody (AB96103)

10% SDS PAGE

All lanes:

Western blot - Anti-CYB5R1 antibody (ab96103) at 1/500 dilution

All lanes:

Hela S3 whole cell lysate at 30 µg

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human CYB5R1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UHQ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYB5R1 also known as NADH-cytochrome b5 reductase is an enzyme that plays an important role in electron transfer. It has a molecular mass of approximately 34 kDa and is found in various tissues like liver and erythrocytes. The enzyme exists in different forms within the cell both membrane-bound and soluble which contribute to its diverse functionality. It catalyzes the reduction of cytochrome b5 using NADH as an electron donor acting as a component of the electron transport chain that facilitates different metabolic processes.
Biological function summary

CYB5R1 participates in lipid metabolism and drug metabolism processes. This enzyme works in concert with cytochrome b5 enhancing metabolic activities involving fatty acid desaturation and elongation. It interacts with a wide array of substrates functioning in critical cellular mechanisms including the reduction of methemoglobin to hemoglobin. Although it does not typically form large complexes CYB5R1's activity is essential for maintaining cellular redox balance.

Pathways

CYB5R1 is involved in pathways such as the electron transport chain and fatty acid metabolism. Its activity influences the proper functioning of the electron transfer system by modulating electron transfer to cytochrome b5 which then influences enzymes like fatty acid desaturase. This modulation helps maintain lipid composition within cellular membranes and contributes to energy production in mitochondria.

CYB5R1 has implications in methemoglobinemia and cancer. Mutations or malfunctions in CYB5R1 can lead to an inability to effectively reduce methemoglobin resulting in methemoglobinemia where hemoglobin cannot carry oxygen efficiently. The enzyme's role in drug metabolism also links it to certain cancer types as alterations in its activity can affect drug efficacy and resistance. Its relationships with proteins like cytochrome P450 highlight CYB5R1’s significance in drug metabolism-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
See full target information CYB5R1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedical reports 6:525-531 PubMed28515911

2017

Expression of tubulin folding cofactor B in mouse hepatic ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Gong,Junyi Wang,Yu Tian,Jing Zhang,Wenjin Liang,Zeming Li,Jidong Yu,Bo Tang,Songqing He
View all publications
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Product promise

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