Rabbit Recombinant Monoclonal CYB5R3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (AB248422), expandable thumbnail](https://content.abcam.com/products/images/cyb5r3-antibody-epr3868-bsa-and-azide-free-ab248422--western-blot-img7865.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (AB248422)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (AB248422), expandable thumbnail](https://content.abcam.com/products/images/cyb5r3-antibody-epr3868-bsa-and-azide-free-ab248422--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21446.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (AB248422)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Alternative names
DIA1, CYB5R3, NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1
Recommended products
Rabbit Recombinant Monoclonal CYB5R3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3868
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248422 is the carrier-free version of Anti-CYB5R3 antibody [EPR3868] ab133247.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein CYB5R3 also known as Cytochrome b5 reductase 3 has a molecular mass of approximately 34 kDa. This enzyme is located in various cellular compartments including the outer mitochondrial membrane and the endoplasmic reticulum. CYB5R3 plays a role in electron transfer by reducing cytochrome b5 using NADH or NADPH as electron donors. This reduction is important for various biosynthesis reactions including fatty acid desaturation and elongation as well as steroid biosynthesis.
- Biological function summary
CYB5R3 contributes to the electron transport chain by facilitating the transfer of reducing equivalents to other systems. This enzyme efficiently elevates the electron flow when functioning within a larger enzymatic complex. CYB5R3 is necessary for maintaining redox balance in cells and supports the synthesis of important biomolecules like cholesterol and lipids due to its involvement in these processes.
- Pathways
CYB5R3 integrates into the fatty acid metabolism and steroid biosynthesis pathways. It effectively partners with cytochrome b5 and other reductases to enable efficient electron flux within these pathways. CYB5R3's activity supports metabolic cooperation with other enzymes such as fatty acid desaturase promoting the production and transformation of essential fatty acids.
- Associated diseases and disorders
Mutations or malfunctions in CYB5R3 lead to conditions such as methemoglobinemia and insulin resistance. Methemoglobinemia results from altered oxygen delivery in the blood due to improper reduction of methemoglobin. In this context the reduced function of CYB5R3 affects the ability of related proteins like hemoglobin to bind oxygen efficiently. Insulin resistance involves metabolic alterations where CYB5R3 acts in coordination with proteins associated with lipid metabolism influencing energy homeostasis and insulin action.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (ab248422), expandable thumbnail](https://content.abcam.com/products/images/cyb5r3-antibody-epr3868-bsa-and-azide-free-ab248422--western-blot-img7865.jpg "Western blot - Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (ab248422)")
Western blot - Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (ab248422)
This data was developed using Anti-CYB5R3 antibody [EPR3868] ab133247, the same antibody clone in a different buffer formulation.
Note: Primary antibody Anti-CYB5R3 antibody [EPR3868] ab133247 is diluted with 1% BSA.
All lanes: Western blot - Anti-CYB5R3 antibody [EPR3868] (Anti-CYB5R3 antibody [EPR3868] ab133247) at 1/1000 dilution
Lane 1: A431 cell lysate at 10 µg
Lane 2: Fetal liver cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: U87 MG cell lysate at 10 µg
Predicted band size: 34 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (ab248422), expandable thumbnail](https://content.abcam.com/products/images/cyb5r3-antibody-epr3868-bsa-and-azide-free-ab248422--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21446.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (ab248422)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R3 antibody [EPR3868] - BSA and Azide free (ab248422)
This data was developed using Anti-CYB5R3 antibody [EPR3868] ab133247, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human testis tissue labelling CYB5R3 with Anti-CYB5R3 antibody [EPR3868] ab133247 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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