Skip to main content

Rabbit Polyclonal CYC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYC1 aa 150 to C-terminus.

Be the first to review this product! Submit a review

Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human CYC1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P08574
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Target data

Function

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Polyclonal CYC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYC1 aa 150 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human CYC1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P08574
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

The electron transport functionality of CYC1 is essential for ATP production through oxidative phosphorylation. CYC1 is a component of the cytochrome bc1 complex which includes other subunits such as Cytochrome b and the Rieske iron-sulfur protein. This interaction allows CYC1 to transfer electrons within the complex driving the conversion of biochemical energy stored in reduced substrates into ATP.

Activity summary

CYC1 also known as Cytochrome c1 is a subunit of the cytochrome bc1 complex or complex III located in the inner mitochondrial membrane. It has a molecular mass of approximately 30 kDa. CYC1 is expressed in various tissues with high energy demands such as heart skeletal muscle and brain. As part of complex III CYC1 plays an important role in the electron transport chain facilitating the transfer of electrons from ubiquinol to cytochrome c contributing to the generation of a proton gradient across the mitochondrial membrane.

Pathways

CYC1 takes part in the mitochondrial oxidative phosphorylation pathway a critical process for cellular respiration. It also interacts within electron transfer pathways alongside related proteins such as Cytochrome c and Complex IV. Their coordinated actions ensure efficient energy production and maintain the mitochondrial function. Its proper function within these pathways is necessary for maintaining metabolic balance in cells.

Associated diseases and disorders

Defects in CYC1 can connect to metabolic anomalies like mitochondrial diseases and cardiomyopathies. Mutations affecting the CYC1 subunit may impair oxidative phosphorylation leading to energy deficits in cells. Additionally disruptions in CYC1 functionality have been linked to disorders involving Cytochrome b another component of the cytochrome bc1 complex contributing to the pathogenesis of various mitochondrial myopathies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (ab224044), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (ab224044)

    Immunohistochemistry analysis of human skeletal tissue labelling CYC1 with ab224044 at 1/200.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (ab224044), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (ab224044)

    Immunohistochemistry analysis of human kidney tissue labelling CYC1 with ab224044 at 1/200.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (ab224044), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (ab224044)

    Immunohistochemistry analysis of human duodenum tissue labelling CYC1 with ab224044 at 1/200.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (ab224044), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (ab224044)

    Immunohistochemistry analysis of human cerebral cortex tissue labelling CYC1 with ab224044 at 1/200.

  • Western blot - Anti-CYC1 antibody (ab224044), expandable thumbnail

    Western blot - Anti-CYC1 antibody (ab224044)

    All lanes: Western blot - Anti-CYC1 antibody (AB224044) at 1/100 dilution

    All lanes: MCF7 (human breast adenocarcinoma cell line) cell lysate

    Predicted band size: 35 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CYC1 antibody (ab224044), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CYC1 antibody (ab224044)

    PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for CYC1 (green) using ab224044 at 4 μg/ml in ICC/IF.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com