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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal CYC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYC1 aa 150 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Cow | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.
Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Cytochrome c-1, CYC1
Rabbit Polyclonal CYC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYC1 aa 150 to C-terminus.
Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Cytochrome c-1, CYC1
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
The electron transport functionality of CYC1 is essential for ATP production through oxidative phosphorylation. CYC1 is a component of the cytochrome bc1 complex which includes other subunits such as Cytochrome b and the Rieske iron-sulfur protein. This interaction allows CYC1 to transfer electrons within the complex driving the conversion of biochemical energy stored in reduced substrates into ATP.
CYC1 also known as Cytochrome c1 is a subunit of the cytochrome bc1 complex or complex III located in the inner mitochondrial membrane. It has a molecular mass of approximately 30 kDa. CYC1 is expressed in various tissues with high energy demands such as heart skeletal muscle and brain. As part of complex III CYC1 plays an important role in the electron transport chain facilitating the transfer of electrons from ubiquinol to cytochrome c contributing to the generation of a proton gradient across the mitochondrial membrane.
CYC1 takes part in the mitochondrial oxidative phosphorylation pathway a critical process for cellular respiration. It also interacts within electron transfer pathways alongside related proteins such as Cytochrome c and Complex IV. Their coordinated actions ensure efficient energy production and maintain the mitochondrial function. Its proper function within these pathways is necessary for maintaining metabolic balance in cells.
Defects in CYC1 can connect to metabolic anomalies like mitochondrial diseases and cardiomyopathies. Mutations affecting the CYC1 subunit may impair oxidative phosphorylation leading to energy deficits in cells. Additionally disruptions in CYC1 functionality have been linked to disorders involving Cytochrome b another component of the cytochrome bc1 complex contributing to the pathogenesis of various mitochondrial myopathies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemistry analysis of human skeletal tissue labelling CYC1 with ab224044 at 1/200.
Immunohistochemistry analysis of human kidney tissue labelling CYC1 with ab224044 at 1/200.
Immunohistochemistry analysis of human duodenum tissue labelling CYC1 with ab224044 at 1/200.
Immunohistochemistry analysis of human cerebral cortex tissue labelling CYC1 with ab224044 at 1/200.
All lanes: Western blot - Anti-CYC1 antibody (AB224044) at 1/100 dilution
All lanes: MCF7 (human breast adenocarcinoma cell line) cell lysate
Predicted band size: 35 kDa
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for CYC1 (green) using ab224044 at 4 μg/ml in ICC/IF.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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