AB224044

Anti-CYC1 antibody

Name: Anti-CYC1 antibody (ab224044) | Abcam
Brand: Abcam Limited
SKU: AB224044

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(2 Publications)

Rabbit Polyclonal CYC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CYC1 aa 150 to C-terminus.

View Alternative Names

Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Cytochrome c-1, CYC1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (AB224044)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (AB224044)

Immunohistochemistry analysis of human cerebral cortex tissue labelling CYC1 with ab224044 at 1/200.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (AB224044)

Immunohistochemistry analysis of human skeletal tissue labelling CYC1 with ab224044 at 1/200.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (AB224044)

Immunohistochemistry analysis of human kidney tissue labelling CYC1 with ab224044 at 1/200.

Immunocytochemistry/ Immunofluorescence - Anti-CYC1 antibody (AB224044)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CYC1 antibody (AB224044)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for CYC1 (green) using ab224044 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (AB224044)

Immunohistochemistry analysis of human duodenum tissue labelling CYC1 with ab224044 at 1/200.

Supplier Data

Western blot - Anti-CYC1 antibody (AB224044)

All lanes:

Western blot - Anti-CYC1 antibody (ab224044) at 1/100 dilution

All lanes:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CYC1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P08574

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYC1 also known as Cytochrome c1 is a subunit of the cytochrome bc1 complex or complex III located in the inner mitochondrial membrane. It has a molecular mass of approximately 30 kDa. CYC1 is expressed in various tissues with high energy demands such as heart skeletal muscle and brain. As part of complex III CYC1 plays an important role in the electron transport chain facilitating the transfer of electrons from ubiquinol to cytochrome c contributing to the generation of a proton gradient across the mitochondrial membrane.

Biological function summary

The electron transport functionality of CYC1 is essential for ATP production through oxidative phosphorylation. CYC1 is a component of the cytochrome bc1 complex which includes other subunits such as Cytochrome b and the Rieske iron-sulfur protein. This interaction allows CYC1 to transfer electrons within the complex driving the conversion of biochemical energy stored in reduced substrates into ATP.

Pathways

CYC1 takes part in the mitochondrial oxidative phosphorylation pathway a critical process for cellular respiration. It also interacts within electron transfer pathways alongside related proteins such as Cytochrome c and Complex IV. Their coordinated actions ensure efficient energy production and maintain the mitochondrial function. Its proper function within these pathways is necessary for maintaining metabolic balance in cells.

Defects in CYC1 can connect to metabolic anomalies like mitochondrial diseases and cardiomyopathies. Mutations affecting the CYC1 subunit may impair oxidative phosphorylation leading to energy deficits in cells. Additionally disruptions in CYC1 functionality have been linked to disorders involving Cytochrome b another component of the cytochrome bc1 complex contributing to the pathogenesis of various mitochondrial myopathies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.

See full target information CYC1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Analytical cellular pathology (Amsterdam) 2024:6624283 PubMed39624161

2024

N-Acetylcysteine Treats Spinal Cord Injury by Inhibiting Astrocyte Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhang,Chaoxi Qin,Fei Meng,Xiaopeng Han,Xing Guo

International journal of molecular medicine 42:3291-3299 PubMed30280186

2018

Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tianzhu Lu,Hongjing Liu,Ganhua You

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CYC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (AB224044)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (AB224044)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (AB224044)

Immunocytochemistry/ Immunofluorescence - Anti-CYC1 antibody (AB224044)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYC1 antibody (AB224044)

Western blot - Anti-CYC1 antibody (AB224044)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise