Anti-Cyclin A1 antibody [EPR24373-146] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 antibody [EPR24373-146] - BSA and Azide free (AB282119)

This data was developed using ab270940, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling Cyclin A1 with ab270940 at 1/2000 (0.231 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control : no staining on human pancreas (PMID : 9041194). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 antibody [EPR24373-146] - BSA and Azide free (AB282119)

This data was developed using ab270940, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Cyclin A1 with ab270940 at 1/2000 (0.231 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on the pachytene spermatocytes of human testis (PMID : 14696091). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 antibody [EPR24373-146] - BSA and Azide free (AB282119)

This data was developed using ab270940, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling Cyclin A1 with ab270940 at 1/2000 (0.231 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control : no staining on human cardiac muscle (PMID : 9041194). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) was used.

• WB

Lab

Western blot - Anti-Cyclin A1 antibody [EPR24373-146] - BSA and Azide free (AB282119)

This data was developed using ab270940, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Cyclin A1 antibody [EPR24373-146] (<a href='/en-us/products/primary-antibodies/cyclin-a1-antibody-epr24373-146-ab270940'>ab270940</a>) at 1/1000 dilution

All lanes:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 52 kDa

false

• WB

Lab

Western blot - Anti-Cyclin A1 antibody [EPR24373-146] - BSA and Azide free (AB282119)

This data was developed using ab270940, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 10 seconds

All lanes:

Western blot - Anti-Cyclin A1 antibody [EPR24373-146] (<a href='/en-us/products/primary-antibodies/cyclin-a1-antibody-epr24373-146-ab270940'>ab270940</a>) at 1/5000 dilution

Lane 1:

His-tagged human cyclinA1 recombinant protein (aa51-223)

Lane 2:

His-tagged human cyclinA2 recombinant protein (aa1-431)

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 28 kDa

false