Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (AB185619)

Immunohistochemical analysis of paraffin-embedded human colon cancer, labeling Cyclin A1 + Cyclin A2 with ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining of human colonic adenocarcinoma cells is observed. Counterstained with Hematoxylin.
Negative control obtained using PBS instead of ab185619 and secondary antibody only.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (AB185619)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Cyclin A1 + Cyclin A2 using ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining of spermatocytes of the human testis is observed. Counterstained with Hematoxylin.
Negative control obtained using PBS instead of ab185619 and secondary antibody only.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (AB185619)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 49 kDa,52 kDa

Observed band size: 49 kDa,52 kDa

false

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (AB185619)

Cyclin A1 + Cyclin A2 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab185619 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab185619 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : K562 whole cell extract 10 μg (Input).

Lane 2 : ab185619 IP in K562 whole cell extract.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab185619 in K562 whole cell extract.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds

All lanes:

Immunoprecipitation - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)

false

• WB

Supplier Data

Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (AB185619)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 49 kDa,52 kDa

Observed band size: 49 kDa,52 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (AB185619)

Recombinant fragment of Human Cyclin A1 protein contains aa261-450 with His-Tag & GST-tag.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/20000 dilution

All lanes:

Recombinant fragment of Human Cyclin A1 protein at 0.01 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 49 kDa,52 kDa

Observed band size: 47 kDa

false

Exposure time: 2s

• WB

Supplier Data

Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (AB185619)

Recombinant full length Cyclin A2 protein contains aa1-432 with His-Tag.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/2000 dilution

All lanes:

Full length Human Cyclin A2 recombinant protein at 0.01 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 49 kDa,52 kDa

Observed band size: 50 kDa

false

Exposure time: 5s

• WB

CiteAb

Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (AB185619)

Cyclin A1 + Cyclin A2 western blot using anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] ab185619. Publication image and figure legend from Xiao, H., Jiang, Y., et al., 2020, Aging (Albany NY), PubMed 32392178.

ab185619 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab185619 please see the product overview.

MXRA5 participates in the regulation of cell cycle via MAPK pathway. (A, B) Downregulation of proteins involved the cell cyle regulation (CDK2, CDK4, Cyclin D1 and Cyclin A1/2) in the WPMY-1 cells was revealed by Western blot analysis. GAPDH was used as a control and values of statistical data shown were mean ± SD of triplicate measurements and repeated three times with similar results. Statistical significance was calculated using ANOVA and ** means p < 0.01. (C, D) Western blot analysis for phosphorylated and total ERK1/2 as well as p38 MAPK (phosphorylated (p) and total (t). GAPDH was used as a control and ** means p < 0.01.

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