Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] - BSA and Azide free (AB240294)

Immunohistochemical analysis of paraffin-embedded human colon cancer, labeling Cyclin A1 + Cyclin A2 with ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining of human colonic adenocarcinoma cells is observed. Counterstained with Hematoxylin.
Negative control obtained using PBS instead of ab185619 and secondary antibody only.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185619).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] - BSA and Azide free (AB240294)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Cyclin A1 + Cyclin A2 using ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining of spermatocytes of the human testis is observed. Counterstained with Hematoxylin.
Negative control obtained using PBS instead of ab185619 and secondary antibody only.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185619).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] - BSA and Azide free (AB240294)

Cyclin A1 + Cyclin A2 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab185619 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab185619 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : K562 whole cell extract 10 µg (Input).

Lane 2 : ab185619 IP in K562 whole cell extract.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab185619 in K562 whole cell extract.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185619).

All lanes:

Immunoprecipitation - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (<a href='/en-us/products/primary-antibodies/cyclin-a1-cyclin-a2-antibody-epr18054-ab185619'>ab185619</a>)

false