Anti-Cyclin A2 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody (AB137769)

Immunohistochemical analysis of paraffin embedded NCIN87 xenograft, labelling Cyclin A using ab137769 at 1/500 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody (AB137769)

Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells labelling Cyclin A2 protein at nucleus using ab137769 at a 1/500 dilution at RT for 15 min (Green). phalloidin, a cytoskeleton marker was stained with anti-phalloidin antibody at a 1/100 dilution (Red). Scale bar= 10 µm.

• WB

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Western blot - Anti-Cyclin A2 antibody (AB137769)

All lanes:

Western blot - Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate at 30 µg

Predicted band size: 49 kDa

false

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody (AB137769)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 48 kDa

false

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody (AB137769)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

Lane 1:

IMR32 whole cell extract at 30 µg

Lane 2:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 48 kDa

false

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody (AB137769)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

All lanes:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 48 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Cyclin A2 antibody (AB137769)

ab137769 immunoprecipitates Cyclin A2 protein in IP experiments. IP Sample : 1000 μg 293T whole cell lysate. A.) 30 μg 293T whole cell lysate. B.) Control with 2.5 μg of preimmune rabbit IgG. C.) Immunoprecipitation of Cyclin A2 protein by 2.5 μg of ab137769 10% SDS-PAGE : The immunoprecipitated Cyclin A2 protein was detected by ab137769 diluted at 1/1000.

All lanes:

Immunoprecipitation - Anti-Cyclin A2 antibody (ab137769)

Predicted band size: 49 kDa

false

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody (AB137769)

All lanes:

Western blot - Anti-Cyclin A2 antibody (ab137769) at 1/1000 dilution

All lanes:

Neuro2A whole cell lysate at 30 µg

Predicted band size: 49 kDa,52 kDa

false