AB39

Anti-Cyclin A2 antibody [E67.1]

Name: Anti-Cyclin A2 antibody [E67.1] (ab39) | Abcam
Brand: Abcam Limited
SKU: AB39
Price: 565 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(8 Publications)

Mouse Monoclonal Cyclin A2 antibody. Suitable for IP and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Full Length Protein corresponding to Cow CCNA2.

View Alternative Names

CCN1, CCNA, CCNA2, Cyclin-A2, Cyclin-A, Cyclin A

1 Images

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Immunoprecipitation - Anti-Cyclin A2 antibody [E67.1] (AB39)

Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2.

All lanes:

Immunoprecipitation - Anti-Cyclin A2 antibody [E67.1] (ab39)

Predicted band size: 49 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

E67.1

Isotype

IgG2a

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Full Length Protein corresponding to Cow CCNA2. The exact immunogen used to generate this antibody is proprietary information.

P30274

Epitope

The epitope for this antibody was mapped by phage selection - selected phage contained the sequence LDYP. This would place the epitope within the region spanning amino acids 144 - 148 of human Cyclin A2.Cyclin As that contain the epitopeCow A2Human A2Hamster A2Mouse A2 Cyclin As that do not contain the epitope Chicken A2Frog A2Human A1Mouse A1Frog A1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2.5 µg/mL", "IP-species-notes": "<p></p>" } } }

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Product details

Few other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.

See full target information CCNA2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36692284

2023

Beta human papillomavirus 8E6 promotes alternative end joining.

Applications

Unspecified application

Species

Unspecified reactive species

Changkun Hu,Taylor Bugbee,Rachel Palinski,Ibukun A Akinyemi,Michael T McIntosh,Thomas MacCarthy,Sumita Bhaduri-McIntosh,Nicholas Wallace

Parasitology research 119:2563-2577 PubMed32548739

2020

Besnoitia besnoiti-driven endothelial host cell cycle alteration.

Applications

Unspecified application

Species

Unspecified reactive species

Zahady D Velásquez,Sara Lopez-Osorio,Learta Pervizaj-Oruqaj,Susanne Herold,Carlos Hermosilla,Anja Taubert

International journal of molecular sciences 20: PubMed31731785

2019

The Effects of Meldonium on the Renal Acute Ischemia/Reperfusion Injury in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Siniša Đurašević,Maja Stojković,Ljiljana Bogdanović,Slađan Pavlović,Slavica Borković-Mitić,Ilijana Grigorov,Desanka Bogojević,Nebojša Jasnić,Tomislav Tosti,Saša Đurović,Jelena Đorđević,Zoran Todorović

Nature communications 10:5125 PubMed31719531

2019

ZEB1/NuRD complex suppresses TBC1D2b to stimulate E-cadherin internalization and promote metastasis in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Roxsan Manshouri,Etienne Coyaud,Samrat T Kundu,David H Peng,Sabrina A Stratton,Kendra Alton,Rakhee Bajaj,Jared J Fradette,Rosalba Minelli,Michael D Peoples,Alessandro Carugo,Fengju Chen,Christopher Bristow,Jeffrey J Kovacs,Michelle C Barton,Tim Heffernan,Chad J Creighton,Brian Raught,Don L Gibbons

Journal of Alzheimer's disease : JAD 33:249-63 PubMed22941973

2012

Characteristics of TBS-extractable hyperphosphorylated tau species: aggregation intermediates in rTg4510 mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Naruhiko Sahara,Michael DeTure,Yan Ren,Abdul-Shukkur Ebrahim,Dongcheul Kang,Joshua Knight,Christiane Volbracht,Jan Torleif Pedersen,Dennis W Dickson,Shu-Hui Yen,Jada Lewis

The Journal of cell biology 191:1285-97 PubMed21173116

2010

Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories.

Applications

Species

Human

Xin Quan Ge,J Julian Blow

Cellular signalling 23:555-65 PubMed21111810

2010

Serine phosphorylation regulates disabled-1 early isoform turnover independently of Reelin.

Applications

Species

Unspecified reactive species

Zhihua Gao,Roseline Godbout

The Journal of biological chemistry 283:5477-85 PubMed18077453

2007

Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network.

Applications

ICC/IF

Species

Mouse

Ka-Kei Ho,Alexandra A Anderson,Erika Rosivatz,Eric W-F Lam,Rüdiger Woscholski,David J Mann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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