Anti-Cyclin A2 antibody [EPR17351]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on some tumor cells in Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on germinal center cells of Human tonsil tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [EPR17351]- Loading Control (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab181591 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150120) at 1/500 dilution.
-ve control 2 : Anti-alpha Tubulin antibody [EPR17351]- Loading Control (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on a proportion of epithelial cells in mouse endometrial tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Cyclin A2 with ab181591 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on a proportion of epithelial cells in rat colon tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Blocking/Dilution buffer : 5% NFDM/TBST.

The smaller band is due to a tissue-specific splice variant, PMID 22745723.

All lanes:

Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/2000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 22 kDa,49 kDa,84 kDa

Observed band size: 110 kDa,18 kDa,22 kDa,50 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/20000 dilution

All lanes:

Human tonsil lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/20000 dilution

All lanes:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Blocking/Dilution buffer : 5% NFDM/TBST.

Cyclin A2 is down regulated at the G2/M phase.

All lanes:

Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/20000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; Untreated, asynchronous cells (<a href='/en-us/products/unavailable/hela-cell-cycle-lysates-thymidine-treated-nocodazole-treated-untreated-asynchronous-control-ab136811'>ab136811</a>) at 1 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; G1/S arrested cells (thymidine treatment) (<a href='/en-us/products/unavailable/hela-cell-cycle-lysates-thymidine-treated-nocodazole-treated-untreated-asynchronous-control-ab136811'>ab136811</a>) at 1 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; G2/M arrested cells (sequential thymidine and nocodazole treatments) (<a href='/en-us/products/unavailable/hela-cell-cycle-lysates-thymidine-treated-nocodazole-treated-untreated-asynchronous-control-ab136811'>ab136811</a>) at 1 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated or Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Blocking/Dilution buffer : 5% NFDM/TBST.

The smaller band is due to a tissue-specific splice variant, PMID 22745723.

All lanes:

Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/2000 dilution

All lanes:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody [EPR17351] (AB181591)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cyclin A2 antibody [EPR17351] (ab181591) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Exposure time: 3s