AB215436

Anti-Cyclin B1 antibody

Name: Anti-Cyclin B1 antibody (ab215436) | Abcam
Brand: Abcam Limited
SKU: AB215436
Price: 510 USD
Availability: InStock

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(10 Publications)

Rabbit Polyclonal Cyclin B1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ccnb1 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CCNB, CCNB1, G2/mitotic-specific cyclin-B1

6 Images

Flow Cytometry (Intracellular) - Anti-Cyclin B1 antibody (AB215436)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cyclin B1 antibody (AB215436)

Intracellular flow cytometric analysis of HeLa cells labeling Cyclin B1with ab215436 at 1/100 dilution for 60 minutesat room temperature, followed by incubation with aGoat Anti-Rabbit IgG (H+L) Alexa Fluor 488 conjugated secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody (AB215436)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody (AB215436)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Cyclin B1 with ab215436 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining

Supplier Data

Western blot - Anti-Cyclin B1 antibody (AB215436)

All lanes:

Western blot - Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution

All lanes:

HeLa cell lysates

Predicted band size: 48 kDa

false

Supplier Data

Western blot - Anti-Cyclin B1 antibody (AB215436)

All lanes:

Western blot - Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution

All lanes:

U937 cell lysates

Predicted band size: 48 kDa

false

Supplier Data

Western blot - Anti-Cyclin B1 antibody (AB215436)

All lanes:

Western blot - Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution

All lanes:

K562 cell lysates

Predicted band size: 48 kDa

false

Supplier Data

Western blot - Anti-Cyclin B1 antibody (AB215436)

All lanes:

Western blot - Anti-Cyclin B1 antibody (ab215436) at 1/100 dilution

All lanes:

U251 cell lysates

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

Synthetic Peptide within Mouse Ccnb1 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P24860

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

See full target information CCNB1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20230654 PubMed37197357

2023

Effect of miR-499-5p/SOX6 axis on atrial fibrosis in rats with atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyuan Han,Shunda Wang,Zhijun Yong,Xueting Zhang,Xuanqi Wang,Penghua You

International journal of molecular sciences 23: PubMed35897737

2022

The Pt(-pr-thiosal)2 and BCL1 Leukemia Lymphoma: Antitumor Activity In Vitro and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Zana Besser Silconi,Vesna Rosic,Sasa Benazic,Gordana Radosavljevic,Marina Mijajlovic,Jelena Pantic,Zoran R Ratkovic,Gordana Radic,Aleksandar Arsenijevic,Marija Milovanovic,Nebojsa Arsenijevic,Jelena Milovanovic

Journal of clinical laboratory analysis 36:e24609 PubMed35838025

2022

The intercorrelation among CCT6A, CDC20, CCNB1, and PLK1 expressions and their clinical value in papillary thyroid carcinoma prognostication.

Applications

IHC

Species

Human

Wei Peng,Wenqiang Li,Xiaoyong Zhang,Weili Cen,Yanan Liu

Oncology letters 24:261 PubMed35765271

2022

MicroRNA-409-5p inhibits cell proliferation, and induces G/M phase arrest and apoptosis by targeting DLGAP5 in ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Li,Ji Lin,Jianfen Huang,Zhuoying Chen,Qunying Sheng,Fang Yang,Xue Yang,Xiaojie Cui

Folia histochemica et cytobiologica 60:101-109 PubMed35257357

2022

Psoralen inhibits the proliferation and promotes apoptosis through endoplasmic reticulum stress in human osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shubo Li,Hongqin Tu

Frontiers in pharmacology 12:764015 PubMed34744744

2021

Aberrant ROS Mediate Cell Cycle and Motility in Colorectal Cancer Cells Through an Oncogenic CXCL14 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zeng,Mei Li,Jun-Yu Xu,Heng Xiao,Xian Yang,Jiao-Xiu Fan,Kang Wu,Shuang Chen

International journal of medical sciences 18:2910-2919 PubMed34220318

2021

Effect of MT2A on apoptosis and proliferation in HL60 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Qing Pan,Min Niu,Shu-Min Liu,Yu-Xia Bao,Kai Yang,Xiao-Bo Ma,Liang He,Yi-Xun Li,Jie-Xian Cao,Xi Zhang,Yan Du

Cellular signalling 85:110049 PubMed34082012

2021

YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Su,Guanzhi Fan,Jin Huang,Xueshan Qiu

Journal of Cancer 12:1990-1999 PubMed33753997

2021

Brucine inhibits proliferation of glioblastoma cells by targeting the G-quadruplexes in the c-Myb promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaochu Liu,Qunhui Wang,Chuanqi Lv,Ziqiang Liu,Haijun Gao,Yong Chen,Gang Zhao

Cell death and differentiation 28:1493-1511 PubMed33230261

2020

Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Canu,Sara Donzelli,Andrea Sacconi,Federica Lo Sardo,Claudio Pulito,Noa Bossel,Anna Di Benedetto,Paola Muti,Claudio Botti,Eytan Domany,Silvio Bicciato,Sabrina Strano,Yosef Yarden,Giovanni Blandino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin B1 antibody

Flow Cytometry (Intracellular) - Anti-Cyclin B1 antibody (AB215436)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody (AB215436)

Western blot - Anti-Cyclin B1 antibody (AB215436)

Western blot - Anti-Cyclin B1 antibody (AB215436)

Western blot - Anti-Cyclin B1 antibody (AB215436)

Western blot - Anti-Cyclin B1 antibody (AB215436)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

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