AB212977

Anti-Cyclin B1 antibody [CCNB1/1098]

Name: Anti-Cyclin B1 antibody [CCNB1/1098] (ab212977) | Abcam
Brand: Abcam Limited
SKU: AB212977
Price: 495 USD
Availability: InStock
Rating: 2 (1 reviews)

(1 Review)

(3 Publications)

Mouse Monoclonal Cyclin B1 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCNB1.

View Alternative Names

CCNB, CCNB1, G2/mitotic-specific cyclin-B1

7 Images

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Immunocytochemistry - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunofluorescence Analysis of formaldehyde-fixed MCF-7 cells stained with 5 ug/mL ab212977 (red) and DNA is stained with Hoechst (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling Cyclin B1 with ab212977 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Cyclin B1 with ab212977 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testicular carcinoma tissue labeling Cyclin B1 with ab212977 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling Cyclin B1 with ab212977 at 1 μg/ml.

Unknown

Immunocytochemistry - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunofluorescence Analysis of formaldehyde-fixed MCF-7 cells stained with 5 ug/mL ab212977 (red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human endometrial carcinoma tissue labeling Cyclin B1 with ab212977 at 1 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CCNB1/1098

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CCNB1.

P14635

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 minutes at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab212977 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

See full target information CCNB1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:17605 PubMed36266453

2022

Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Sook Shin,Akshara Thakore,Yuko Tada,Albert J Pedroza,Gentaro Ikeda,Ian Y Chen,Doreen Chan,Kevin J Jaatinen,Shin Yajima,Eric M Pfrender,Masashi Kawamura,Phillip C Yang,Joseph C Wu,Eric A Appel,Michael P Fischbein,YJoseph Woo,Yasuhiro Shudo

American journal of cancer research 11:4175-4198 PubMed34659882

2021

Experimental and clinical evidence suggests that GRPEL2 plays an oncogenic role in HCC development.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Chun Lai,Qian-Qian Zhu,Jun Xu,Wen-Jin Zhang

Cellular physiology and biochemistry : internation 46:2271-2283 PubMed29734192

2018

MiR-27b Promotes Muscle Development by Inhibiting MDFI Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lianjie Hou,Jian Xu,Yiren Jiao,Huaqin Li,Zhicheng Pan,Junli Duan,Ting Gu,Chingyuan Hu,Chong Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin B1 antibody [CCNB1/1098]

Immunocytochemistry - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunocytochemistry - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [CCNB1/1098] (AB212977)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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