AB18221

Anti-Cyclin B1 antibody [V92.1]

Name: Anti-Cyclin B1 antibody [V92.1] (ab18221) | Abcam
Brand: Abcam Limited
SKU: AB18221
Price: 500 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal Cyclin B1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Human CCNB1.

View Alternative Names

CCNB, CCNB1, G2/mitotic-specific cyclin-B1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (AB18221)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (AB18221)

ICC/IF image of ab18221 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18221, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

V92.1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Full Length Protein corresponding to Human CCNB1. The exact immunogen used to generate this antibody is proprietary information.

P14635

Specificity

V92.1 can immunoprecipitate active CDK1/cyclin B1 complexes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

See full target information CCNB1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 16:4185-4192 PubMed30250533

2018

Identification of key pathways and genes in lung carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Jin,Xingang Liu,Zhen Zhang,Yinghui Guan,Ren Xv,Jun Li

PLoS pathogens 13:e1006721 PubMed29121649

2017

Spatiotemporal dynamics of HSV genome nuclear entry and compaction state transitions using bioorthogonal chemistry and super-resolution microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Eiki Sekine,Nora Schmidt,David Gaboriau,Peter O'Hare

Oncogene 21:8089-104 PubMed12444545

2002

Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew P Garner,Claire R Weston,Daniel E Todd,Kathryn Balmanno,Simon J Cook

Experimental cell research 262:37-48 PubMed11120603

2000

Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.

Applications

Unspecified application

Species

Unspecified reactive species

Y Minemoto,J Gannon,M Masutani,H Nakagama,T Sasagawa,M Inoue,Y Masamune,K Yamashita

View all publications

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