AB3488

Anti-Cyclin B1 (phospho S126) antibody

Name: Anti-Cyclin B1 (phospho S126) antibody (ab3488) | Abcam
Brand: Abcam Limited
SKU: AB3488
Price: 535 USD
Availability: InStock

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(2 Publications )

Rabbit Polyclonal Cyclin B1 phospho S126 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CCNB1 pS126 aa 100-150.

1 Images

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Western blot - Anti-Cyclin B1 (phospho S126) antibody (AB3488)

All lanes:

Western blot - Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

H23 whole cell lysate at 50 µg

Lane 3:

Hepatocarcinoma (Hep3b) cell lysate at 50 µg

Lane 4:

Glioblastoma (T98G) cell lysate at 50 µg

Lane 5:

B cell lymphoblast (Daudi) cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution

Predicted band size: 48 kDa

true

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CCNB1 pS126 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P14635

Specificity

<p>This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

See full target information CCNB1 pS126

Alternative Names

CCNB, CCNB1, G2/mitotic-specific cyclin-B1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:734-745 PubMed40836083

2025

Targeting G1-S-checkpoint-compromised cancers with cyclin A/B RxL inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Shilpa Singh,Catherine E Gleason,Min Fang,Yasmin N Laimon,Vishal Khivansara,Shanhai Xie,Yavuz T Durmaz,Aniruddha Sarkar,Kenneth Ngo,Varunika Savla,Yixiang Li,Muhannad Abu-Remaileh,Xinyue Li,Marie-Anais Locquet,Bishma Tuladhar,Ranya Odeh,Frances Hamkins-Indik,Daphne He,Miles W Membreno,Meisam Nosrati,Nathan N Gushwa,Siegfried S F Leung,Breena Fraga-Walton,Luis Hernandez,Miguel P Baldomero,Bryan M Lent,David Spellmeyer,Joshua F Luna,Dalena Hoang,Yuliana Gritsenko,Manesh Chand,Megan K DeMart,Sammy Metobo,Chinmay Bhatt,Justin A Shapiro,Kai Yang,Nathan J Dupper,Andrew T Bockus,Jinshu Fang,Ramesh Bambal,Peadar Cremin,John G Doench,James B Aggen,Li-Fen Liu,Bernard Levin,Evelyn W Wang,Iolanda Vendrell,Roman Fischer,Benedikt Kessler,Prafulla C Gokhale,Sabina Signoretti,Alexander Spektor,Constantine Kreatsoulas,Marie Evangelista,Rajinder Singh,David J Earp,Deepak Nijhawan,Pablo D Garcia,Matthew G Oser

Aging cell 16:575-584 PubMed28345297

2017

Residual Cdk1/2 activity after DNA damage promotes senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Müllers,Helena Silva Cascales,Kamila Burdova,Libor Macurek,Arne Lindqvist

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin B1 (phospho S126) antibody

Western blot - Anti-Cyclin B1 (phospho S126) antibody (AB3488)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Alternative Names

Publications (2)

Product promise