AB133439

Anti-Cyclin B1 (phospho S126) antibody [EPR3670]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal Cyclin B1 phospho S126 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CCNB, CCNB1, G2/mitotic-specific cyclin-B1

1 Images

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Western blot - Anti-Cyclin B1 (phospho S126) antibody [EPR3670] (AB133439)

All lanes:

Western blot - Anti-Cyclin B1 (phospho S126) antibody [EPR3670] (ab133439) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Lysate of HeLa cells treated with Nocodazole at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3670

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects Cyclin B phosphorylated at Serine 126.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

See full target information CCNB1 phospho S126

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Parasitology research 119:2563-2577 PubMed32548739

2020

Besnoitia besnoiti-driven endothelial host cell cycle alteration.

Applications

Unspecified application

Species

Unspecified reactive species

Zahady D Velásquez,Sara Lopez-Osorio,Learta Pervizaj-Oruqaj,Susanne Herold,Carlos Hermosilla,Anja Taubert

Scientific reports 9:12496 PubMed31467333

2019

Toxoplasma gondii-induced host cellular cell cycle dysregulation is linked to chromosome missegregation and cytokinesis failure in primary endothelial host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zahady D Velásquez,Iván Conejeros,Camilo Larrazabal,Katharina Kerner,Carlos Hermosilla,Anja Taubert

Molecular medicine (Cambridge, Mass.) 25:23 PubMed31138113

2019

CKD-602, a topoisomerase I inhibitor, induces apoptosis and cell-cycle arrest and inhibits invasion in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sungha Lee,Jung Yoon Ho,Jing Jing Liu,Hyewon Lee,Jae Young Park,Minwha Baik,Minji Ko,Seon Ui Lee,Youn Jin Choi,Soo Young Hur

Cell death & disease 9:448 PubMed29670107

2018

Inhibition of cancer progression by a novel trans-stilbene derivative through disruption of microtubule dynamics, driving G2/M arrest, and p53-dependent apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Pravat Kumar Parida,Barun Mahata,Abhisek Santra,Sohini Chakraborty,Zhumur Ghosh,Sanghamitra Raha,Anup Kumar Misra,Kaushik Biswas,Kuladip Jana

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin B1 (phospho S126) antibody [EPR3670]

Western blot - Anti-Cyclin B1 (phospho S126) antibody [EPR3670] (AB133439)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (4)

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