AB131502

Anti-Cyclin B1 (phospho S147) antibody

Name: Anti-Cyclin B1 (phospho S147) antibody (ab131502) | Abcam
Brand: Abcam Limited
SKU: AB131502
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Cyclin B1 phospho S147 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CCNB1 pS147 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CCNB, CCNB1, G2/mitotic-specific cyclin-B1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 (phospho S147) antibody (AB131502)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 (phospho S147) antibody (AB131502)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelled with ab131502 at 1/50 dilution (left) and ab131502 preincubated with blocking peptide (right).

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 (phospho S147) antibody (AB131502)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 (phospho S147) antibody (AB131502)

Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131502 at 1/100 dilution.

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Western blot - Anti-Cyclin B1 (phospho S147) antibody (AB131502)

All lanes:

Western blot - Anti-Cyclin B1 (phospho S147) antibody (ab131502) at 1/500 dilution

Lane 1:

Untreated MDA cell lysate

Lane 2:

Lysate of MDA cells treated with anisomycin

Predicted band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CCNB1 pS147 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P14635

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab131502 was purified by epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

See full target information CCNB1 pS147

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:10353-10367 PubMed40896797

2025

Cardiac Homing Peptide-Functionalized Polymeric Nanoparticles Suppressing SHP1 Alleviate Acute Myocardial Infarction Injury by Promoting Efferocytosis and Inhibiting Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Pan,Guihao Chen,Xiaoli Zhuang,Fei Li,Yuejin Yang

Developmental cell 53:42-59.e11 PubMed32109383

2020

Lamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei and Myocardial Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Han,Sangita Choudhury,Jocelyn D Mich-Basso,Niyatie Ammanamanchi,Balakrishnan Ganapathy,Sangita Suresh,Mugdha Khaladkar,Jennifer Singh,Rene Maehr,Daniel A Zuppo,Junhyong Kim,James H Eberwine,Samuel K Wyman,Yijen L Wu,Bernhard Kühn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin B1 (phospho S147) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 (phospho S147) antibody (AB131502)

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 (phospho S147) antibody (AB131502)

Western blot - Anti-Cyclin B1 (phospho S147) antibody (AB131502)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Product promise