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Rabbit Recombinant Monoclonal Cyclin B2/CCNB2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 18 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/80

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/500

Notes

-

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/100

Notes

Recommended for human and rat but not mouse. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100

Notes

Recommended for human and rat but not mouse. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Alternative names

Recommended products

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Rabbit Recombinant Monoclonal Cyclin B2/CCNB2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 18 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

R17985

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622), expandable thumbnail

    Western blot - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cyclin B2/CCNB2 antibody [R17985] (AB185622) at 1/1000 dilution

    Lane 1: SW480 (Human colon adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Cyclin B2/CCNB2 with ab185622 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on PC-12 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab185622 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colon adenocarcinoma cell line) cells labeling Cyclin B2/CCNB2 with ab185622 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab185622 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Western blot - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622), expandable thumbnail

    Western blot - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cyclin B2/CCNB2 antibody [R17985] (AB185622) at 1/5000 dilution

    Lane 1: A549 (Human lung carcinoma) whole cell lysate at 20 µg

    Lane 2: Mouse testis lysate at 20 µg

    Lane 3: Rat testis lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/1000 dilution

    Predicted band size: 24 kDa, 45 kDa

    Observed band size: 24 kDa, 45 kDa

    Exposure time: 3min

  • Western blot - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622), expandable thumbnail

    Western blot - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cyclin B2/CCNB2 antibody [R17985] (AB185622) at 1/1000 dilution

    Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 20 µg

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/1000 dilution

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Cyclin B2/CCNB2 with ab185622 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on normal Human colon epithelium is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622), expandable thumbnail

    Immunoprecipitation - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622)

    Cyclin B2/CCNB2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab185622 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab185622 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).

    Lane 2: ab185622 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185622 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time:10 seconds

    All lanes: Immunoprecipitation - Anti-Cyclin B2/CCNB2 antibody [R17985] (AB185622)

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622)

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Cyclin B2/CCNB2 with ab185622 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on tumor cells of Human colon epithelium is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B2/CCNB2 antibody [R17985] (ab185622)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Cyclin B2/CCNB2 with ab185622 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on spermatocytes of rat testis is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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