AB10839

Anti-Cyclin B2/CCNB2 antibody [X121.10]

Name: Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839) | Abcam
Brand: Abcam Limited
SKU: AB10839
Rating: 5 (1 reviews)

(1 Review)

(6 Publications)

Mouse Monoclonal Cyclin B2/CCNB2 antibody. Suitable for WB and reacts with Xenopus laevis samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Xenopus laevis G2/mitotic-specific cyclin-B2.

View Alternative Names

G2/mitotic-specific cyclin-B2, ccnb2

1 Images

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Western blot - Anti-Cyclin B2/CCNB2 antibody [X121.10] (AB10839)

All lanes:

Western blot - Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839) at 1 µg/mL

Lane 1:

Interphase Xenopus egg extracts (cyclin B destroyed)

Lane 2:

Mitotic (CSF) Xenopus egg extracts (cyclin B present)

Predicted band size: 45 kDa

Observed band size: 38 kDa,40 kDa,54 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

X121.10

Isotype

IgG1

Carrier free

Reacts with

Xenopus laevis

Applications

applications

Immunogen

Native Full Length Protein corresponding to Xenopus laevis G2/mitotic-specific cyclin-B2.

P13351

Epitope

The epitope for X121 lies within a region spanning the amino acids 1-112.

Specificity

ab10839 blots only cyclin B2/CCNB2, but cross reacts strongly with two other proteins. However, these can easily be distinguished from cyclins since they are present in interphase extracts and run well below the cyclins on SDS PAGE gels.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Xenopus laevis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

See full target information G2/mitotic-specific cyclin-B2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:751 PubMed37880655

2023

Decoding meningioma heterogeneity and neoplastic cell-macrophage interaction through single-cell transcriptome profiling across pathological grades.

Applications

Unspecified application

Species

Unspecified reactive species

Hailang Fan,Lairong Song,Jian Fan,Junpeng Ma,Xiaojie Li,Junting Zhang,Jian Hu,Zhen Wu,Dake Zhang,Liang Wang

Experimental and therapeutic medicine 19:1779-1788 PubMed32104233

2020

Integrative module analysis of HCC gene expression landscapes.

Applications

Unspecified application

Species

Unspecified reactive species

Hongshi Li,Ning Wei,Yi Ma,Xiaozhou Wang,Zhiqiang Zhang,Shuang Zheng,Xi Yu,Shuang Liu,Lijie He

Biological research 51:21 PubMed29980225

2018

Electroacupuncture ameliorate learning and memory by improving N-acetylaspartate and glutamate metabolism in APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ruhui Lin,Long Li,Yingzheng Zhang,Sheng Huang,Shangjie Chen,Jiao Shi,Peiyuan Zhuo,Hao Jin,Zuanfang Li,Weilin Liu,Zhifu Wang,Lidian Chen,Jing Tao

Cell cycle (Georgetown, Tex.) 6:732-8 PubMed17361104

2007

Emi2 at the crossroads: where CSF meets MPF.

Applications

Unspecified application

Species

Unspecified reactive species

David V Hansen,Joseph R Pomerening,Matthew K Summers,Julie J Miller,James E Ferrell,Peter K Jackson

Molecular biology of the cell 13:4388-400 PubMed12475960

2002

Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan D Moore,Sally Kornbluth,Tim Hunt

Experimental cell research 253:413-21 PubMed10585264

1999

Activation of Xenopus eggs by the kinase inhibitor 6-DMAP suggests a differential regulation of cyclin B and p39(mos) proteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

J F Bodart,D Béchard,M Bertout,J Gannon,A Rousseau,J P Vilain,S Flament

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin B2/CCNB2 antibody [X121.10]

Western blot - Anti-Cyclin B2/CCNB2 antibody [X121.10] (AB10839)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise