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AB18250

Anti-Cyclin B2/CCNB2 antibody [X29.2]

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(3 Reviews)

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(19 Publications)

Mouse Monoclonal Cyclin B2/CCNB2 antibody. Suitable for IHC-P, Flow Cyt and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Full Length Protein corresponding to Xenopus laevis G2/mitotic-specific cyclin-B2.

View Alternative Names

G2/mitotic-specific cyclin-B2, CCNB2

2 Images
Flow Cytometry - Anti-Cyclin B2/CCNB2 antibody [X29.2] (AB18250)
  • Flow Cyt

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Flow Cytometry - Anti-Cyclin B2/CCNB2 antibody [X29.2] (AB18250)

Overlay histogram showing HeLA cells stained with ab18250 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18250, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B2/CCNB2 antibody [X29.2] (AB18250)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B2/CCNB2 antibody [X29.2] (AB18250)

IHC image of ab18250 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18250, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

X29.2

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Xenopus laevis G2/mitotic-specific cyclin-B2. The exact immunogen used to generate this antibody is proprietary information.

P13351

Specificity

Reacts with most cyclin Bs.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for the control of the cell cycle at the G2/M (mitosis) transition.
See full target information G2/mitotic-specific cyclin-B2
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:113782 PubMed38358892

2024

Unraveling the interplay between PKA inhibition and Cdk1 activation during oocyte meiotic maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Santoni,Ferdinand Meneau,Nabil Sekhsoukh,Sandrine Castella,Tran Le,Marika Miot,Enrico Maria Daldello

Aging 13:9838-9858 PubMed33744866

2021

lncRNA SNHG4 modulates colorectal cancer cell cycle and cell proliferation through regulating miR-590-3p/CDK1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyi Zhou,Fengbo Tan,Qian Pei,Chenglong Li,Yuan Zhou,Yuqiang Li,Haiping Pei

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 134:111139 PubMed33360155

2020

Olive leaf extract impairs mitochondria by pro-oxidant activity in MDA-MB-231 and OVCAR-3 cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Reyes Benot-Dominguez,Maria Grazia Tupone,Vanessa Castelli,Michele d'Angelo,Elisabetta Benedetti,Massimiliano Quintiliani,Benedetta Cinque,Iris Maria Forte,Maria Grazia Cifone,Rodolfo Ippoliti,Barbara Barboni,Antonio Giordano,Annamaria Cimini

Aging 12:3025-3041 PubMed32045367

2020

Obg-like ATPase 1 (OLA1) overexpression predicts poor prognosis and promotes tumor progression by regulating P21/CDK2 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shanzhou Huang,Chuanzhao Zhang,Chengjun Sun,Yuchen Hou,Yixi Zhang,Nga Lei Tam,Zekang Wang,Jia Yu,Bowen Huang,Hongkai Zhuang,Zixuan Zhou,Zuyi Ma,Zhonghai Sun,Xiaoshun He,Qi Zhou,Baohua Hou,Linwei Wu

Oncology letters 19:2568-2574 PubMed32194761

2020

Effect of miR-205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wang,Haomin Zhang,Kai Jiao,Chunyang Zhao,Hailong Liu,Qinghong Meng,Zhao Wang,Chunling Feng,Yuanchun Li

Cancers 11: PubMed31717700

2019

Synergism Through WEE1 and CHK1 Inhibition in Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Ghelli Luserna Di Rorà,Matteo Bocconcelli,Anna Ferrari,Carolina Terragna,Samantha Bruno,Enrica Imbrogno,Neil Beeharry,Valentina Robustelli,Martina Ghetti,Roberta Napolitano,Gabriella Chirumbolo,Giovanni Marconi,Cristina Papayannidis,Stefania Paolini,Chiara Sartor,Giorgia Simonetti,Timothy J Yen,Giovanni Martinelli

Cell cycle (Georgetown, Tex.) 18:395-412 PubMed30730241

2019

PKCβ1 regulates meiotic cell cycle in mouse oocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Yun Yi,Qiu-Xia Liang,Tie-Gang Meng,Jian Li,Ming-Zhe Dong,Yi Hou,Ying-Chun Ouyang,Chun-Hui Zhang,Heide Schatten,Qing-Yuan Sun,Jie Qiao,Wei-Ping Qian

Molecular carcinogenesis 58:293-304 PubMed30334580

2018

Exportin-T promotes tumor proliferation and invasion in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Lin,Yuchen Hou,Shanzhou Huang,Ziming Wang,Chengjun Sun,Zekang Wang,Xiaoshun He,Nga Lei Tam,Chenglin Wu,Linwei Wu

The Journal of cell biology 217:3901-3911 PubMed30097513

2018

Cyclin B2 can compensate for Cyclin B1 in oocyte meiosis I.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Ji-Xin Tang,Jin-Mei Cheng,Bian Hu,Yu-Qian Wang,Batool Aalia,Xiao-Yu Li,Cheng Jin,Xiu-Xia Wang,Shou-Long Deng,Yan Zhang,Su-Ren Chen,Wei-Ping Qian,Qing-Yuan Sun,Xing-Xu Huang,Yi-Xun Liu

Journal of hematology & oncology 11:99 PubMed30068368

2018

Targeting WEE1 to enhance conventional therapies for acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Ghelli Luserna Di Rorà,Neil Beeharry,Enrica Imbrogno,Anna Ferrari,Valentina Robustelli,Simona Righi,Elena Sabattini,Maria Vittoria Verga Falzacappa,Chiara Ronchini,Nicoletta Testoni,Carmen Baldazzi,Cristina Papayannidis,Maria Chiara Abbenante,Giovanni Marconi,Stefania Paolini,Sarah Parisi,Chiara Sartor,Maria Chiara Fontana,Serena De Matteis,Ilaria Iacobucci,Pier Giuseppe Pelicci,Michele Cavo,Timothy J Yen,Giovanni Martinelli
View all publications
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