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Rabbit Recombinant Monoclonal Cyclin B3 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B3 antibody [EPR24921-9] - BSA and Azide free (AB309952), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B3 antibody [EPR24921-9] - BSA and Azide free (AB309952), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B3 antibody [EPR24921-9] - BSA and Azide free (AB309952), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Cyclins are positive regulatory subunits of the cyclin-dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle, notably via their destruction during cell division. Its tissue specificity suggest that it may be required during early meiotic prophase I.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Cyclin B3 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR24921-9

Purification technique

Affinity purification Protein A

Light chain type

kappa

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab309952 is the carrier free version of Anti-Cyclin B3 antibody [EPR24921-9] ab309951.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B3 antibody [EPR24921-9] - BSA and Azide free (ab309952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B3 antibody [EPR24921-9] - BSA and Azide free (ab309952)

    This data was developed using Anti-Cyclin B3 antibody [EPR24921-9] ab309951, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded formalin-fixed paraffin-embedded normal human cerebellum tissue labeling G2/mitotic-specific cyclin-B3 with Anti-Cyclin B3 antibody [EPR24921-9] ab309951 at 1µg/ml followed by a secondary antibody.

    Counterstained with Hematoxylin.

    Negative control: secondary antibody only.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B3 antibody [EPR24921-9] - BSA and Azide free (ab309952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B3 antibody [EPR24921-9] - BSA and Azide free (ab309952)

    This data was developed using Anti-Cyclin B3 antibody [EPR24921-9] ab309951, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded formalin-fixed paraffin-embedded normal human liver tissue labeling G2/mitotic-specific cyclin-B3 with Anti-Cyclin B3 antibody [EPR24921-9] ab309951 at 1µg/ml followed by a secondary antibody.

    Counterstained with Hematoxylin.

    Negative control: secondary antibody only.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B3 antibody [EPR24921-9] - BSA and Azide free (ab309952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B3 antibody [EPR24921-9] - BSA and Azide free (ab309952)

    This data was developed using Anti-Cyclin B3 antibody [EPR24921-9] ab309951, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded formalin-fixed paraffin-embedded normal human liver testis labeling G2/mitotic-specific cyclin-B3 with Anti-Cyclin B3 antibody [EPR24921-9] ab309951 at 1µg/ml followed by a secondary antibody.

    Counterstained with Hematoxylin.

    Negative control: secondary antibody only.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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