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AB85927

Anti-Cyclin C antibody

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(7 Publications)

Rabbit Polyclonal Cyclin C antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CCNC aa 200 to C-terminus.

View Alternative Names

Cyclin-C, SRB11 homolog, hSRB11, CCNC

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin C antibody (AB85927)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin C antibody (AB85927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Cyclin C with ab85927 at 1/1000 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-Cyclin C antibody (AB85927)
  • IP

Unknown

Immunoprecipitation - Anti-Cyclin C antibody (AB85927)

Detection of Human Cyclin C in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85927 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Cyclin C antibody (ab85927)

Predicted band size: 33 kDa

false

Western blot - Anti-Cyclin C antibody (AB85927)
  • WB

Unknown

Western blot - Anti-Cyclin C antibody (AB85927)

All lanes:

Western blot - Anti-Cyclin C antibody (ab85927) at 0.1 µg/mL

Lane 1:

Panc1 whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

H2009 whole cell lysate at 50 µg

Predicted band size: 33 kDa

Observed band size: 33 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human CCNC aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P24863

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.
See full target information CCNC
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 14:306-317 PubMed36741255

2023

C4orf47 contributes to the dormancy of pancreatic cancer under hypoxic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Shinjiro Nagao,Hideya Onishi,Makoto Kawamoto,Shogo Masuda,Lin Na,Shinji Morisaki,Naoya Iwamoto,Yutaka Yamada,Satoko Koga,Shu Ichimiya,Kazunori Nakayama,Akira Imaizumi,Kinichi Nakashima,Yoshinao Oda,Masafumi Nakamura

Nature communications 13:754 PubMed35136067

2022

A Mediator-cohesin axis controls heterochromatin domain formation.

Applications

Unspecified application

Species

Unspecified reactive species

Judith H I Haarhuis,Robin H van der Weide,Vincent A Blomen,Koen D Flach,Hans Teunissen,Laureen Willems,Thijn R Brummelkamp,Benjamin D Rowland,Elzo de Wit

Nature communications 12:2693 PubMed33976158

2021

An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Liu,Tianyu Tao,Shihua Liu,Xia Yang,Xuwei Chen,Jiaer Liang,Ruohui Hong,Wenting Wang,Yi Yang,Xiaoyi Li,Youhong Zhang,Quanfeng Li,Shujun Liang,Haocheng Yu,Yun Wu,Xinyu Guo,Yan Lai,Xiaofan Ding,Hongyu Guan,Jueheng Wu,Xun Zhu,Jie Yuan,Jun Li,Shicheng Su,Mengfeng Li,Xiuyu Cai,Junchao Cai,Han Tian

Cancer cell international 21:19 PubMed33407495

2021

Emodin regulates cell cycle of non-small lung cancer (NSCLC) cells through hyaluronan synthase 2 (HA2)-HA-CD44/receptor for hyaluronic acid-mediated motility (RHAMM) interaction-dependent signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhu Li,Shengbo Jin,Yang Cao,Jian Xu,Shendong Zhu,Zheng Li

The Journal of biological chemistry 294:12743-12753 PubMed31270208

2019

Lipogenic SREBP-1a/c transcription factors activate expression of the iron regulator hepcidin, revealing cross-talk between lipid and iron metabolisms.

Applications

Unspecified application

Species

Unspecified reactive species

Alus M Xiaoli,Ziyi Song,Fajun Yang

Journal of Cancer 10:3389-3396 PubMed31293642

2019

Overexpression of miR-15b Promotes Resistance to Sunitinib in Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Lu,Yanmin Li,Hui Wen,Chenchen Feng

PloS one 8:e72496 PubMed23967313

2013

Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene.

Applications

Unspecified application

Species

Unspecified reactive species

Aya Fukuda,Miho Shimada,Tomoyoshi Nakadai,Ken Nishimura,Koji Hisatake
View all publications
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