Anti-Cyclin D1 antibody - C-terminal

• WB

Supplier Data

Western blot - Anti-Cyclin D1 antibody - C-terminal (AB185241)

All lanes:

Western blot - Anti-Cyclin D1 antibody - C-terminal (ab185241) at 1/25 dilution

All lanes:

MCF7 cell lysate

Predicted band size: 34 kDa

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• WB

Supplier Data

Western blot - Anti-Cyclin D1 antibody - C-terminal (AB185241)

False colour image of Western blot : Anti-Cyclin D1 antibody - C-terminal staining at 1/25 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab185241 was shown to bind specifically to Cyclin D1. A band was observed at 35 kDa in wild-type A549 cell lysates with no signal observed at this size in ccnd1 knockout cell line ab286759 (knockout cell lysate ab300213). To generate this image, wild-type and ccnd1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Cyclin D1 antibody - C-terminal (ab185241) at 1/25 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human CCND1 knockout A549 cell lysate (ab300213) at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 35 kDa

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