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AB62151

Anti-Cyclin D1 (phospho T286) antibody

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(8 Publications)

Rabbit Polyclonal Cyclin D1 phospho T286 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CCND1 phospho T286 aa 200 to C-terminus.

View Alternative Names

BCL1, PRAD1, CCND1, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1

1 Images
Western blot - Anti-Cyclin D1 (phospho T286) antibody (AB62151)
  • WB

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Western blot - Anti-Cyclin D1 (phospho T286) antibody (AB62151)

All lanes:

Western blot - Anti-Cyclin D1 (phospho T286) antibody (ab62151) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells treated with EGF (200ng/ml, 30mins)

Lane 2:

Extracts from Jurkat cells treated with EGF (200ng/ml, 30mins) with immunizing phosphopeptide

Predicted band size: 34 kDa

Observed band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CCND1 phospho T286 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P24385

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the CCND1 gene acts as a regulatory component of the cyclin D1-CDK4 (DC) complex, which phosphorylates and inhibits retinoblastoma (RB) protein family members, including RB1, regulating the cell cycle during the G(1)/S transition. Phosphorylation of RB1 allows the transcription factor E2F to dissociate from the RB/E2F complex, enabling transcription of E2F target genes responsible for G(1) phase progression. In early G(1) phase, it hypophosphorylates RB1. Cyclin D-CDK4 complexes integrate various mitogenic and antimitogenic signals. The protein also serves as a substrate for SMAD3, phosphorylating it in a cell-cycle-dependent manner to repress its transcriptional activity, and forms part of the cyclin D1/CDK4/CDKN1B complex necessary for nuclear translocation and activity. Additionally, it exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters, independent of the cell cycle. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CCND1 phospho T286
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 14:e70056 PubMed40130745

2025

MFGE-8, a Corona Protein on Extracellular Vesicles, Mediates Self-Renewal and Survival of Human Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Youngseok Lee,Hyojin Kim,Heeseok Yoon,Seunghyun Cho,Jeongjun Kim,Jihun Lee,Sang-Hun Choi,Hyesun Cho,Dong-Hun Woo,Jung-Hyuck Park,Choongseong Han,Jong-Hoon Kim

Cellular and molecular life sciences : CMLS 81:215 PubMed38739166

2024

Prenatal treatment with preimplantation factor improves early postnatal neurogenesis and cognitive impairments in a mouse model of Down syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Manon Moreau,Rodolphe Dard,Amélia Madani,Janany Kandiah,Nadim Kassis,Jessica Ziga,Héloïse Castiglione,Solenn Day,Thomas Bourgeois,Boris Matrot,François Vialard,Nathalie Janel

American journal of cancer research 13:2188-2200 PubMed37293180

2023

Eukaryotic initiation factor 3b regulates the development and progression of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhui Li,Jianhua Zhao,Zhikun Yuan,Dehan Yuan,Mao Fang,Minru Xie,Minghan Xia

Journal of immunology research 2022:7372202 PubMed36106050

2022

FBXL16 Promotes Endometrial Progesterone Resistance via PP2A /Cyclin D1 Axis in Ishikawa.

Applications

Unspecified application

Species

Unspecified reactive species

Haoen Liu,Li Han,Liyan Zhong,Xiaodan Zhuang,Yan Peng

Bioengineered 12:9869-9884 PubMed34719326

2021

Essential meiotic structure-specific endonuclease1 () promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiguo Guo,Erbo Liang,Wei Li,Leilei Jiang,Fachao Zhi

The Journal of biological chemistry 292:10131-10141 PubMed28446612

2017

CDK4 protein is degraded by anaphase-promoting complex/cyclosome in mitosis and reaccumulates in early G phase to initiate a new cell cycle in HeLa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huabo Chen,Xiaowei Xu,Guopeng Wang,Boyan Zhang,Gang Wang,Guangwei Xin,Junjun Liu,Qing Jiang,Hongyin Zhang,Chuanmao Zhang

Oncology reports 29:2079-87 PubMed23467929

2013

Let-7: a regulator of the ERα signaling pathway in human breast tumors and breast cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Sun,Sida Qin,Chong Fan,Chongwen Xu,Ning Du,Hong Ren

Cell cycle (Georgetown, Tex.) 11:1705-13 PubMed22510561

2012

Evidence for cell cycle suppression and microRNA regulation of cyclin D1 during anoxia exposure in turtles.

Applications

WB

Species

Unspecified reactive species

Kyle K Biggar,Kenneth B Storey
View all publications
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