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AB256538

Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] - BSA & Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Cyclin D1 phospho T286 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant fragment, Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

BCL1, PRAD1, CCND1, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1, G1/S-specific cyclin-D2, CCND2

1 Images
Dot Blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] - BSA & Azide free (AB256538)
  • Dot

Unknown

Dot Blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235] - BSA & Azide free (AB256538)

Dot blot analysis using ab230818 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/100,000 dilution.

Lane 1 : CCND1 non-phospho peptide (aa279-294)

Lane 2 : CCND1 (phospho T286) peptide (aa284-294)

Lane 3 : CCND2 (phospho T280) peptide (aa278-288

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230818).

  • Unconjugated

    Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22328-235

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Anti-Cyclin D1 (phospho T286) + Cyclin D2 (phospho T280) antibody [EPR22328-235]

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Primary Antibodies

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Primary Antibodies

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Reactivity data

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Product details

ab256538 is the carrier-free version of ab230818.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 33854235, PubMed : 8114739, PubMed : 8302605). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 8114739, PubMed : 8302605). Hypophosphorylates RB1 in early G(1) phase (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 8114739, PubMed : 8302605). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 8302605). Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity (PubMed : 15241418). Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed : 9106657). Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (PubMed : 16569215, PubMed : 18417529).
See full target information CCND1 phospho T286

Additional targets

CCND2 phospho T280
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101791 PubMed39426375

2024

Schwann cell-secreted frizzled-related protein 1 dictates neuroinflammation and peripheral nerve degeneration after neurotrauma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyun Yao,Lingchi Kong,Yi Qiao,David Brand,Juehong Li,Zhiwen Yan,Song Guo Zheng,Yun Qian,Cunyi Fan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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