Rabbit Recombinant Monoclonal Cyclin D2 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Mouse, Human, Mouse, Rat samples.
Images
![Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (AB308259), expandable thumbnail](https://content.abcam.com/products/images/cyclin-d2-antibody-epr27468-62-bsa-and-azide-free-ab308259--western-blot-img429585.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (AB308259)")
![Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (AB308259), expandable thumbnail](https://content.abcam.com/products/images/cyclin-d2-antibody-epr27468-62-bsa-and-azide-free-ab308259--western-blot-img409735.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (AB308259)")
![Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (AB308259), expandable thumbnail](https://content.abcam.com/products/images/cyclin-d2-antibody-epr27468-62-bsa-and-azide-free-ab308259--western-blot-img409733.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (AB308259)")
![Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (AB308259), expandable thumbnail](https://content.abcam.com/products/images/cyclin-d2-antibody-epr27468-62-bsa-and-azide-free-ab308259--western-blot-img409734.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (AB308259)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | Flow Cyt (Intra) | ICC/IF | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Alternative names
G1/S-specific cyclin-D2, Cyl-2, Ccnd2
Recommended products
Rabbit Recombinant Monoclonal Cyclin D2 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Mouse, Human, Mouse, Rat samples.
Alternative names
G1/S-specific cyclin-D2, Cyl-2, Ccnd2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR27468-62
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259), expandable thumbnail](https://content.abcam.com/products/images/cyclin-d2-antibody-epr27468-62-bsa-and-azide-free-ab308259--western-blot-img429585.jpg "Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259)")
Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
We recommend using a higher sensitive ECL substrate to increase the band intensity.
This data was developed using Anti-Cyclin D2 antibody [EPR27468-62] ab308258, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Cyclin D2 antibody [EPR27468-62] (Anti-Cyclin D2 antibody [EPR27468-62] ab308258) at 1/1000 dilution
Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: U-2 OS (Human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg
Lane 3: Human heart tissue lysate at 20 µg
Lane 4: Human thyroid tissue lysate at 20 µg
Lane 5: Mouse heart tissue lysate at 20 µg
Lane 6: Mouse thyroid tissue lysate at 20 µg
Lane 7: Rat heart tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 31 kDa
Exposure time: 40s
![Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259), expandable thumbnail](https://content.abcam.com/products/images/cyclin-d2-antibody-epr27468-62-bsa-and-azide-free-ab308259--western-blot-img409735.jpg "Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259)")
Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259)
This data was developed using 308258, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS. Lysates at 20 µg per lane.
The samples were run on a Bis-Tris gel under reducing conditions.
Western blot: Anti-Cyclin D2 antibody (Anti-Cyclin D2 antibody [EPR27468-62] ab308258) staining at 1/1000 dilution, shown in green; Mouse anti-Tubulin antibody DM1A (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red.
In Western blot, Anti-Cyclin D2 antibody [EPR27468-62] ab308258 was shown to bind specifically to Cyclin D2. Target of interest was observed at 31 kDa in wild-type 293T cell lysates (lane 1) with no signal observed at this size in Cyclin D2 knockout cell line Human CCND2 (Cyclin D2) knockout HEK-293T cell line ab267318(Cyclin D2 knockout cell lysate Human CCND2 (Cyclin D2) knockout HEK-293T cell lysate ab257875) (lane 2). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.All lanes: Western blot - Anti-Cyclin D2 antibody [EPR27468-62] (Anti-Cyclin D2 antibody [EPR27468-62] ab308258) at 1/1000 dilution
Lane 1: Wild-type 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: CCND2 knockout 293T whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: C2C12 (mouse myoblast) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 31 kDa
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS. Lysates at 20 µg per lane.
The samples were run on a Bis-Tris gel under reducing conditions.
Western blot: Anti-Cyclin D2 antibody (Anti-Cyclin D2 antibody [EPR27468-62] ab308258) staining at 1/1000 dilution, shown in green; Mouse anti-Tubulin antibody DM1A (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red.
In Western blot, Anti-Cyclin D2 antibody [EPR27468-62] ab308258 was shown to bind specifically to Cyclin D2. Target of interest was observed at 31 kDa in wild-type 293T cell lysates (lane 1) with no signal observed at this size in Cyclin D2 knockout cell line Human CCND2 (Cyclin D2) knockout HEK-293T cell line ab267318(Cyclin D2 knockout cell lysate Human CCND2 (Cyclin D2) knockout HEK-293T cell lysate ab257875) (lane 2). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.![Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259), expandable thumbnail](https://content.abcam.com/products/images/cyclin-d2-antibody-epr27468-62-bsa-and-azide-free-ab308259--western-blot-img409733.jpg "Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259)")
Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259)
This data was developed using 308258, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with mouse CCND3 and CCND1.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.#8 seconds
Exposure time: 8 secondsAll lanes: Western blot - Anti-Cyclin D2 antibody [EPR27468-62] (Anti-Cyclin D2 antibody [EPR27468-62] ab308258) at 1/1000 dilution
Lane 1: His-tagged mouse CCND2 recombinant protein fragment at 10 ng
Lane 2: His-tagged mouse CCND3 recombinant protein at 10 ng
Lane 3: MYC/DDK -tagged mouse CCND1 recombinant protein at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 32 kDa
Observed band size: 31 kDa
Exposure time: 8s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with mouse CCND3 and CCND1.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.#8 seconds
Exposure time: 8 seconds![Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259), expandable thumbnail](https://content.abcam.com/products/images/cyclin-d2-antibody-epr27468-62-bsa-and-azide-free-ab308259--western-blot-img409734.jpg "Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259)")
Western blot - Anti-Cyclin D2 antibody [EPR27468-62] - BSA and Azide free (ab308259)
This data was developed using 308258, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-Cyclin D2 antibody [EPR27468-62] (Anti-Cyclin D2 antibody [EPR27468-62] ab308258) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: RAW 264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 3: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Lane 5: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 32 kDa
Observed band size: 31 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.
Exposure time: 180 seconds
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