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AB226972

Anti-Cyclin D2 antibody - N-terminal

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(4 Publications)

Rabbit Polyclonal Cyclin D2 antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CCND2.

View Alternative Names

G1/S-specific cyclin-D2, CCND2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D2 antibody - N-terminal (AB226972)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D2 antibody - N-terminal (AB226972)

Paraffin-embedded human cervical carcinoma tissue stained for Cyclin D2 using ab226972 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D2 antibody - N-terminal (AB226972)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D2 antibody - N-terminal (AB226972)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cyclin D2 (green) using ab226972 at 1/500 dilution in ICC/IF. Nuclear counterstained with Hoechst 33342.

Western blot - Anti-Cyclin D2 antibody - N-terminal (AB226972)
  • WB

Supplier Data

Western blot - Anti-Cyclin D2 antibody - N-terminal (AB226972)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-Cyclin D2 antibody - N-terminal (ab226972) at 1/1000 dilution

All lanes:

RD whole cell extract at 30 µg

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CCND2. The exact immunogen used to generate this antibody is proprietary information.

P30279

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed : 18827403, PubMed : 8114739). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed : 18827403, PubMed : 8114739). Hypophosphorylates RB1 in early G(1) phase (PubMed : 18827403, PubMed : 8114739). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed : 18827403, PubMed : 8114739).
See full target information CCND2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 10:620306 PubMed33614501

2021

A Novel Androgen-Induced lncRNA FAM83H-AS1 Promotes Prostate Cancer Progression the miR-15a/CCNE2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Liu,Duocheng Qian,Weidong Zhou,Huiyang Jiang,Zhendong Xiang,Denglong Wu

Cancers 12: PubMed32784654

2020

Human FBXL8 Is a Novel E3 Ligase Which Promotes BRCA Metastasis by Stimulating Pro-Tumorigenic Cytokines and Inhibiting Tumor Suppressors.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Chun Chang,Wayne Hsu,Emily Chia-Yu Su,Chin-Sheng Hung,Jeak Ling Ding

Molecular therapy. Nucleic acids 16:257-271 PubMed30925453

2019

lncRNA TUG1 Promotes Cisplatin Resistance by Regulating CCND2 via Epigenetically Silencing miR-194-5p in Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gan Yu,Hui Zhou,Weimin Yao,Lirong Meng,Bin Lang

Journal of experimental & clinical cancer research 37:230 PubMed30227870

2018

miR-4317 suppresses non-small cell lung cancer (NSCLC) by targeting fibroblast growth factor 9 (FGF9) and cyclin D2 (CCND2).

Applications

Unspecified application

Species

Unspecified reactive species

Xi He,Si-Yuan Chen,Zhao Yang,Jie Zhang,Wei Wang,Mei-Yue Liu,Yi Niu,Xiao-Mei Wei,Hong-Min Li,Wan-Ning Hu,Guo-Gui Sun
View all publications
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Product promise

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