Anti-Cyclin D3/CCND3 antibody [1/Cyclin D3]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [1/Cyclin D3] (AB289546)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Cyclin D3/CCND3 with ab289546 at 1/5000 (0.181 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Sporadically nuclear staining in rat stomach. The section was incubated with ab289546 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. The sample was counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [1/Cyclin D3] (AB289546)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Cyclin D3/CCND3 with ab289546 at 1/5000 (0.181 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Sporadically nuclear staining in mouse stomach. The section was incubated with ab289546 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. The sample was cunterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [1/Cyclin D3] (AB289546)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Cyclin D3/CCND3 with ab289546 at 1/5000 (0.181 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining in rat spleen. The section was incubated with ab289546 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. The sample was counterstained with Hematoxylin.

Secondary antibody only control : secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [1/Cyclin D3] (AB289546)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Cyclin D3/CCND3 with ab289546 at 1/5000 (0.181 μg/ml) dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining in mouse spleen. The section was incubated with ab289546 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Sample was counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-Cyclin D3/CCND3 antibody [1/Cyclin D3] (AB289546)

Cyclin D3/CCND3 was immunoprecipitated from 0.35 mg C2C12 (mouse myoblast) whole cell lysate 10 μg with ab289546 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289546 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.

Lane 1 : C2C12 whole cell lysate 10 μg

Lane 2 : ab289546 IP in C2C12 whole cell lysate

Lane 3 : Mouse IgG2b instead of ab289546 in C2C12 whole cell lysate

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 32 seconds.

All lanes:

Immunoprecipitation - Anti-Cyclin D3/CCND3 antibody [1/Cyclin D3] (ab289546)

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

• WB

Supplier Data

Western blot - Anti-Cyclin D3/CCND3 antibody [1/Cyclin D3] (AB289546)

Blocking and diluting buffer : 5% NFDM/TBST

Exposure times : Lanes 1-7/9-11 : 37 seconds ; Lane 8 : 3 minutes

All lanes:

Western blot - Anti-Cyclin D3/CCND3 antibody [1/Cyclin D3] (ab289546) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 10 µg

Lane 5:

HEL (erythroleukemia erythroblast), whole cell lysate at 10 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 7:

A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 10 µg

Lane 8:

C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

Lane 9:

C2C12 (mouse myoblast), whole cell lysate at 10 µg

Lane 10:

Human lymphoma lysate at 10 µg

Lane 11:

Mouse lymphoma lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false