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AB112034

Anti-Cyclin D3/CCND3 antibody

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(7 Publications)

Rabbit Polyclonal Cyclin D3/CCND3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

G1/S-specific cyclin-D3, CCND3

2 Images
Western blot - Anti-Cyclin D3/CCND3 antibody (AB112034)
  • WB

Unknown

Western blot - Anti-Cyclin D3/CCND3 antibody (AB112034)

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-Cyclin D3/CCND3 antibody (ab112034) at 1 µg/mL

All lanes:

Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa,56 kDa

true

Exposure time: 16min

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D3/CCND3 antibody (AB112034)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D3/CCND3 antibody (AB112034)

ab112034 staining Cyclin D3/CCND3 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab112034 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 69% homology with Cyclin D2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Abcam recommends using milk as the blocking agent (1%)</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Baboon": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed : 8114739). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed : 8114739). Hypophosphorylates RB1 in early G(1) phase (PubMed : 8114739). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed : 8114739). Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed : 16782892). Shows transcriptional coactivator activity with ATF5 independently of CDK4 (PubMed : 15358120).
See full target information G1/S-specific cyclin-D3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:1036401 PubMed36408174

2022

DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Han,Lining Wang,Dongshuo Wang,Luosheng Zhang,Yifeng Bi,Xinlei Zheng,Weibo Liu,Guangjian Bai,Zhenhua Wang,Wei Wan,Yong Ma,Xiaopan Cai,Tielong Liu,Qi Jia

Translational cancer research 11:414-425 PubMed35281418

2022

Knockdown of inhibits the progression of ovarian cancer and is related to the signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fanling Meng,Jing Ding,Wei Xu,Chang Luo,Xihai Chen,Ruichun Zhang,Lin Sui,Yuanlong Hu,Shuang Liu,Guangyue Shi,Yunlong He,Xin Ning,Rong Ma,Ning Huang

Journal of cellular and molecular medicine : PubMed33142041

2020

Inhibition of exosomal miR-24-3p in diabetes restores angiogenesis and facilitates wound repair via targeting PIK3R3.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Xu,Liu Ouyang,Lei He,Yanzhen Qu,Yu Han,Deyu Duan

Aging 12:14978-14989 PubMed32620711

2020

Inhibition of miRNA-152-3p enhances diabetic wound repair via upregulation of PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Xu,Tao Yu,Lei He,Liu Ouyang,Yanzhen Qu,Junjie Zhou,Yu Han,Deyu Duan

Molecular medicine reports 13:1645-52 PubMed26708148

2015

Protein inhibitor of activated STAT xα depresses cyclin D and cyclin D kinase, and contributes to the inhibition of osteosarcoma cell progression.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Wang,Jiangdong Ni,Shuai Yi,Deye Song,Muliang Ding

Molecular medicine reports 11:1905-10 PubMed25378143

2014

Inhibition of osteosarcoma cell progression by MacroH2A via the downregulation of cyclin D and cyclin‑dependent kinase genes.

Applications

Unspecified application

Species

Unspecified reactive species

Pu Yang,Ke Yin,Da Zhong,Qiande Liao,Kanghua Li

Molecular medicine reports 10:1845-50 PubMed25119498

2014

MacroH2A suppresses the proliferation of the B16 melanoma cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Shaorong Lei,Jianhong Long,Jiaguang Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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