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AB183338

Anti-Cyclin D3/CCND3 antibody [SP207]

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal Cyclin D3/CCND3 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples. Cited in 7 publications.

View Alternative Names

G1/S-specific cyclin-D3, CCND3

14 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemical analysis of paraffin-embedded human uterus tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Flow cytometric analysis of K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling Cyclin D3/CCND3 with ab183338 diluted 1/100 (green) compared with a negative control of anti-rabbit IgG (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemical analysis of paraffin-embedded human reactive lymph node tissue labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemical analysis of paraffin-embedded human cervix tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution (3.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemical analysis of paraffin-embedded human skin tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemical analysis of paraffin-embedded human kidney tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Immunohistochemical analysis of paraffin-embedded human placenta tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.

Western blot - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • WB

Lab

Western blot - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : Empty Lane
Lane 3 : CCND3 knockout HAP1 whole cell lysate (20 μg)
Lane 4 : K562 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab183338 observed at 33 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab183338 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE. ab183338 and Mouse anti Vinculin loading control were incubated overnight at 4°C at 100 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)

Predicted band size: 33 kDa

false

Western blot - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)
  • WB

Supplier Data

Western blot - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

All lanes:

Western blot - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338) at 1/100 dilution

All lanes:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP207

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed : 8114739). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed : 8114739). Hypophosphorylates RB1 in early G(1) phase (PubMed : 8114739). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed : 8114739). Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed : 16782892). Shows transcriptional coactivator activity with ATF5 independently of CDK4 (PubMed : 15358120).
See full target information G1/S-specific cyclin-D3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 28:101615 PubMed36592610

2023

Epigenetic regulator KDM4A activates Notch1-NICD-dependent signaling to drive tumorigenesis and metastasis in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Pei,ShengQuan Zhang,Xiaowei Yang,Chunguang Han,Yubo Pan,Jun Li,Zhaorui Wang,Chenyu Sun,Jing Zhang

Cell death & disease 11:1025 PubMed33268767

2020

Long noncoding RNA HOXA-AS2 functions as an oncogene by binding to EZH2 and suppressing LATS2 in acute myeloid leukemia (AML).

Applications

Unspecified application

Species

Unspecified reactive species

Yubin Feng,Shuang Hu,Lanlan Li,Xiaoqing Peng,Feihu Chen

Journal of gynecologic oncology 31:e91 PubMed33078596

2020

High mobility group box 3 promotes cervical cancer proliferation by regulating Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shichao Zhuang,Xiaohui Yu,Ming Lu,Yujiao Li,Ning Ding,Yumei Ding

Journal of ovarian research 13:76 PubMed32622365

2020

Cul4B promotes the progression of ovarian cancer by upregulating the expression of CDK2 and CyclinD1.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Jing Duan,Juan-Hong Zhao,Li-Li Xie

Frontiers in cell and developmental biology 8:142 PubMed32296698

2020

LncRNA NR-104098 Inhibits AML Proliferation and Induces Differentiation Through Repressing EZH2 Transcription by Interacting With E2F1.

Applications

Unspecified application

Species

Unspecified reactive species

Yubin Feng,Shuang Hu,Lanlan Li,Shengpeng Zhang,Jikang Liu,Xiaoling Xu,Meiju Zhang,Tianxi Du,Yan Du,Xiaoqing Peng,Feihu Chen

The American journal of pathology 189:2323-2339 PubMed31640854

2019

Long Noncoding RNA LINC01133 Confers Tumor-Suppressive Functions in Ovarian Cancer by Regulating Leucine-Rich Repeat Kinase 2 as an miR-205 Sponge.

Applications

Unspecified application

Species

Unspecified reactive species

Min Liu,Cuiping Shen,Changxiu Wang

Cancer 119:2765-70 PubMed23657789

2013

Mutation-specific antibody detects mutant BRAFV600E protein expression in human colon carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Frank A Sinicrope,Thomas C Smyrk,David Tougeron,Stephen N Thibodeau,Shalini Singh,Andrea Muranyi,Kandavel Shanmugam,Thomas M Grogan,Steven R Alberts,Qian Shi
View all publications
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Product promise

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