AB55322

Anti-Cyclin D3/CCND3 (phospho T283) antibody

Name: Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322) | Abcam
Brand: Abcam Limited
SKU: AB55322

(1 Review)

(2 Publications)

Rabbit Polyclonal Cyclin D3/CCND3 phospho T283 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human G1/S-specific cyclin-D3 phospho T283 aa 200 to C-terminus.

View Alternative Names

G1/S-specific cyclin-D3, CCND3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 (phospho T283) antibody (AB55322)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 (phospho T283) antibody (AB55322)

This image shows human lung carcinoma tissue stained with ab55322 at 1/50 dilution. The left hand image shows untreated tissue, the right hand image shows tissue treated with the immunising phosphopeptide.

Supplier Data

Western blot - Anti-Cyclin D3/CCND3 (phospho T283) antibody (AB55322)

All lanes:

Western blot - Anti-Cyclin D3/CCND3 (phospho T283) antibody (ab55322)

All lanes:

K562 cell lysate (treated with UV) blocked with phospho peptide.

Predicted band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human G1/S-specific cyclin-D3 phospho T283 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P30281

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed : 8114739). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed : 8114739). Hypophosphorylates RB1 in early G(1) phase (PubMed : 8114739). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed : 8114739). Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed : 16782892). Shows transcriptional coactivator activity with ATF5 independently of CDK4 (PubMed : 15358120).

See full target information G1/S-specific cyclin-D3 phospho T283

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 12: PubMed31861110

2019

Biological Characterization of 8-Cyclopropyl-2-(pyridin-3-yl)thiazolo[5,4-]quinazolin-9(8)-one, a Promising Inhibitor of DYRK1A.

Applications

Unspecified application

Species

Unspecified reactive species

Corinne Fruit,Florence Couly,Rahul Bhansali,Malini Rammohan,Mattias F Lindberg,John D Crispino,Laurent Meijer,Thierry Besson

The Journal of clinical investigation 122:4461-72 PubMed23160194

2012

GSK3β mediates muscle pathology in myotonic dystrophy.

Applications

Species

Human

Karlie Jones,Christina Wei,Polina Iakova,Enrico Bugiardini,Christiane Schneider-Gold,Giovanni Meola,James Woodgett,James Killian,Nikolai A Timchenko,Lubov T Timchenko

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