Anti-Cyclin E1 antibody [CCNE1/2460]

• WB

Lab

Western blot - Anti-Cyclin E1 antibody [CCNE1/2460] (AB238081)

False colour image of Western blot : Anti-Cyclin E1 antibody [CCNE1/2460] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab238081 was shown to bind specifically to Cyclin E1. A band was observed at 47 kDa in wild-type HAP1 cell lysates with no signal observed at this size in CCNE1 CRISPR-Cas9 edited HAP1 cell line. The band observed in the CRISPR-Cas9 edited lysate lane below 47 kDa is likely to represent a truncated form of Cyclin E1. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and CCNE1 CRISPR-Cas9 edited HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-Cyclin E1 antibody [CCNE1/2460] (ab238081) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

CCNE1 CRISPR-Cas9 edited HAP1 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

Human Liver cell lysate at 20 µg

Predicted band size: 47 kDa

Observed band size: 47 kDa

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• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 antibody [CCNE1/2460] (AB238081)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cyclin E1 using ab238081 at 2 μg/ml in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 antibody [CCNE1/2460] (AB238081)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cyclin E1 using ab238081 at 2 μg/ml in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-Cyclin E1 antibody [CCNE1/2460] (AB238081)

All lanes:

Western blot - Anti-Cyclin E1 antibody [CCNE1/2460] (ab238081) at 2 µg/mL

All lanes:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 40 µg

Predicted band size: 47 kDa

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• PepArr

Supplier Data

Peptide Array - Anti-Cyclin E1 antibody [CCNE1/2460] (AB238081)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab238081.Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.