Rabbit Recombinant Monoclonal Cyclin E1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Mouse, Mouse, Rat samples.
Images
![Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (AB317437), expandable thumbnail](https://content.abcam.com/products/images/cyclin-e1-antibody-epr25394-23-bsa-and-azide-free-ab317437--western-blot-img433444.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (AB317437)")
![Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (AB317437), expandable thumbnail](https://content.abcam.com/products/images/cyclin-e1-antibody-epr25394-23-bsa-and-azide-free-ab317437--western-blot-img433443.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (AB317437)")
![Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (AB317437), expandable thumbnail](https://content.abcam.com/products/images/cyclin-e1-antibody-epr25394-23-bsa-and-azide-free-ab317437--western-blot-img433442.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (AB317437)")
![Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (AB317437), expandable thumbnail](https://content.abcam.com/products/images/cyclin-e1-antibody-epr25394-23-bsa-and-azide-free-ab317437--western-blot-img433441.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (AB317437)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (AB317437), expandable thumbnail](https://content.abcam.com/products/images/cyclin-e1-antibody-epr25394-23-bsa-and-azide-free-ab317437--immunocytochemistry-immunofluorescence-img433457.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (AB317437)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Transfected cell lysate - Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Transfected cell lysate - Mouse, Human | Dilution info - | Notes - |
Target data
Function
Essential for the control of the cell cycle at the G1/S (start) transition.
Alternative names
Ccne, G1/S-specific cyclin-E1
Recommended products
Rabbit Recombinant Monoclonal Cyclin E1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Mouse, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25394-23
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody does not cross-react with overexpressed mouse Cyclin E2 (#Q9Z238) by western blot.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317437 is the carrier-free version of Anti-Cyclin E1 antibody [EPR25394-23] ab317436.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437), expandable thumbnail](https://content.abcam.com/products/images/cyclin-e1-antibody-epr25394-23-bsa-and-azide-free-ab317437--western-blot-img433444.jpg "Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)")
Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
Cyclin E1 Western blot staining using rabbit Anti-Cyclin E1 antibody
This data was developed using Anti-Cyclin E1 antibody [EPR25394-23] ab317436, the same antibody clone in a different buffer formulation.
This antibody does not cross-react with mouse Cyclin E2.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Cyclin E1 antibody [EPR25394-23] (Anti-Cyclin E1 antibody [EPR25394-23] ab317436) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg with NFDM/TBST
Lane 2: 293T cells transfected with a mouse Cyclin E1 expression vector containing a His-tag, whole cell lysate at 20 µg with NFDM/TBST
Lane 3: 293T cells transfected with a mouse Cyclin E2 expression vector containing a His-tag, whole cell lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 50 kDa, 124 kDa
Exposure time: 10s
![Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437), expandable thumbnail](https://content.abcam.com/products/images/cyclin-e1-antibody-epr25394-23-bsa-and-azide-free-ab317437--western-blot-img433443.jpg "Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)")
Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
This data was developed using Anti-Cyclin E1 antibody [EPR25394-23] ab317436, the same antibody clone in a different buffer formulation.
The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.Low expression: heart, kidney (PMID: 9840943, PMID: 11687642).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Cyclin E1 antibody [EPR25394-23] (Anti-Cyclin E1 antibody [EPR25394-23] ab317436) at 1/1000 dilution
Lane 1: Rat testis tissue lysate at 20 µg with NFDM/TBST
Lane 2: Rat spleen tissue lysate at 20 µg with NFDM/TBST
Lane 3: Rat heart tissue lysate at 20 µg with NFDM/TBST
Lane 4: Rat kidney tissue lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 50 kDa, 36 kDa
Exposure time: 180s
![Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437), expandable thumbnail](https://content.abcam.com/products/images/cyclin-e1-antibody-epr25394-23-bsa-and-azide-free-ab317437--western-blot-img433442.jpg "Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)")
Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
This data was developed using Anti-Cyclin E1 antibody [EPR25394-23] ab317436, the same antibody clone in a different buffer formulation.
The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.Low expression: lung, heart, kidney (PMID: 9840943, PMID: 11687642).
The identity of the higher molecular weight band at approximately 100 kDa may represent multi-ubiquitylated Cyclin E1 (PMID: 23109421). This has not been confirmed with additional tests.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 70 seconds, lanes 2-6: 180 seconds
All lanes: Western blot - Anti-Cyclin E1 antibody [EPR25394-23] (Anti-Cyclin E1 antibody [EPR25394-23] ab317436) at 1/1000 dilution
Lane 1: Mouse spleen tissue lysate at 20 µg with NFDM/TBST
Lane 2: Mouse placenta tissue lysate at 20 µg with NFDM/TBST
Lane 3: Mouse testis tissue lysate at 20 µg with NFDM/TBST
Lane 4: Mouse lung tissue lysate at 20 µg with NFDM/TBST
Lane 5: Mouse kidney tissue lysate at 20 µg with NFDM/TBST
Lane 6: Mouse heart tissue lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 50 kDa, 100 kDa, 36 kDa
![Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437), expandable thumbnail](https://content.abcam.com/products/images/cyclin-e1-antibody-epr25394-23-bsa-and-azide-free-ab317437--western-blot-img433441.jpg "Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)")
Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
This data was developed using Anti-Cyclin E1 antibody [EPR25394-23] ab317436, the same antibody clone in a different buffer formulation.
The expression of Cyclin E1 is upregulated in response to aphidicolin treatment (PMID: 29995582).
The identity of the higher molecular weight band at approximately 100 kDa may represent multi-ubiquitylated Cyclin E1 (PMID: 23109421). This has not been confirmed with additional tests.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2: 180 seconds, lanes 3-4: 103 seconds
All lanes: Western blot - Anti-Cyclin E1 antibody [EPR25394-23] (Anti-Cyclin E1 antibody [EPR25394-23] ab317436) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST
Lane 2: NIH/3T3 transfected with siRNA specifically targeting Cyclin E1 whole cell lysate at 20 µg with NFDM/TBST
Lane 3: Untreated NIH/3T3 whole cell lysate at 20 µg with NFDM/TBST
Lane 4: NIH/3T3 treated with 10 µg/ml aphidicolin for 24 hours whole cell lysate with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 50 kDa, 100 kDa, 36 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437), expandable thumbnail](https://content.abcam.com/products/images/cyclin-e1-antibody-epr25394-23-bsa-and-azide-free-ab317437--immunocytochemistry-immunofluorescence-img433457.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)")
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
This data was developed using Anti-Cyclin E1 antibody [EPR25394-23] ab317436, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Cyclin E1 with Anti-Cyclin E1 antibody [EPR25394-23] ab317436 at 1/500 (1.004 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).
Confocal image showing nuclear staining in NIH/3T3 cell line (shown in green), and decreased staining in cells during mitosis (indicated by arrowhead). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
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