Anti-Cyclin E1 antibody [SP146] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 antibody [SP146] - BSA and Azide free (AB245729)

This data was developed using the same antibody clone in a different buffer formulation (ab135380).

Immunohistochemistry analysis staining paraffin-embedded sections of human placenta tissue with ab135380 in the following dilutions : Image A : 1 : 100 dilution (2.13 μg/ml), Image B : 1 : 50 dilution (4.26 μg/ml), Image C : 1 : 50 dilution (4.26 μg/ml), Image D : Control. A ready to use secondary was used, and the counterstain was Hematoxylin. Image A and Image B : Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) for 15mins by a pressure cooker. Image C : Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Positive staining on the human placenta. Image A and Image B : The section was incubated with ab135380 at +4°C overnight. Image C : The section was incubated with ab135380 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 antibody [SP146] - BSA and Azide free (AB245729)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labelling Cyclin E1 with ab135380 at 1/100 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab135380)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 antibody [SP146] - BSA and Azide free (AB245729)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling Cyclin E1 with ab135380 at 1/100 dilution (2.60 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer, pH 6.0. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab135380)