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AB326342

Anti-Cyclin E1 (phospho T77) antibody [EPR28889-1] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Cyclin E1 phospho T77 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CCNE, G1/S-specific cyclin-E1

3 Images
Sandwich ELISA - Anti-Cyclin E1 (phospho T77) antibody [EPR28889-1] - BSA and Azide free (Capture) (AB326342)
  • sELISA

Lab

Sandwich ELISA - Anti-Cyclin E1 (phospho T77) antibody [EPR28889-1] - BSA and Azide free (Capture) (AB326342)

HCT 116 + Thymidine : HCT 116 treated first with 2 mM Thymidine for 18 hours, then recovered for 8 hours, and then treated again with 2 mM Thymidine for 18 hours, whole cell lysate. Serial dilutions of lysates were analyzed.
CCNE1 KO HCT 116 + Thymidine : CCNE1 knockout HCT 116 treated first with 2 mM Thymidine for 18 hours, then recovered for 8 hours, and then treated again with 2 mM Thymidine for 18 hours, whole cell lysate. Serial dilutions of lysates were analyzed.

Sandwich ELISA - Anti-Cyclin E1 (phospho T77) antibody [EPR28889-1] - BSA and Azide free (Capture) (AB326342)
  • sELISA

Lab

Sandwich ELISA - Anti-Cyclin E1 (phospho T77) antibody [EPR28889-1] - BSA and Azide free (Capture) (AB326342)

HCT 116 + Thymidine : HCT 116 treated first with 2 mM Thymidine for 18 hours, then recovered for 8 hours, and then treated again with 2 mM Thymidine for 18 hours, whole cell lysate. Serial dilutions of lysates were analyzed.
HCT 116 + Control : untreated HCT 116, whole cell lysate. Serial dilutions of lysates were analyzed.

Sandwich ELISA - Anti-Cyclin E1 (phospho T77) antibody [EPR28889-1] - BSA and Azide free (Capture) (AB326342)
  • sELISA

Lab

Sandwich ELISA - Anti-Cyclin E1 (phospho T77) antibody [EPR28889-1] - BSA and Azide free (Capture) (AB326342)

Indirect ELISA analysis of ab326342 at 2000 ng/mL. The Secondary antibody used was Peroxidase AffiniPure Goat Anti-Rabbit IgG (Fc fragment specific) at 1 : 20000 dilution.

Antigen : Human Cyclin E1 (phospho T77) peptide; Human Cyclin E1 non-phospho peptide; Human Cyclin E2 (phospho T74) peptide.

Antigen concentration : 0.98-1000 ng/mL

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28889-1

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to human Cyclin E1 (phospho T77) and shows no cross-reactivity with human Cyclin E2 (phospho T74). The antibody pair, consisting of ab326342 as the capture and ab244991 as the detector, exhibits no cross-reactivity with human Cyclin E2 (phospho T74).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab326342 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Cyclin E1 (phospho T77). The recommended pair for sandwich ELISA is:

Capture: ab326342, Human Cyclin E1 (phospho T77) Capture Antibody (unconjugated)

Detector: ab244991, Human Cyclin E1 (phospho T77) Detector Antibody (unconjugated)

The reference range value is 0.98-1000 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for the control of the cell cycle at the G1/S (start) transition.
See full target information CCNE1 phospho T77
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com