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AB326630

Anti-Cyclin E1 (phospho T77) antibody [SD2025]

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Rabbit Recombinant Monoclonal Cyclin E1 phospho T77 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples.

View Alternative Names

CCNE, G1/S-specific cyclin-E1

2 Images
Western blot - Anti-Cyclin E1 (phospho T77) antibody [SD2025] (AB326630)
  • WB

Supplier Data

Western blot - Anti-Cyclin E1 (phospho T77) antibody [SD2025] (AB326630)

All lanes : Primary antibody at 1/1000 dilution
Lane 1 : K562 whole cell lysates
Lane 2 : K562 treated with 100nM Calyculin A for 30min whole cell lysates
Lane 3 : 293T whole cell lysates
Lane 4 : 293T treated with 100nM Calyculin A for 30min whole cell lysates
Lane 5 : 293T treated with Lambda Protein Phosphatase for 30min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 47 kDa
Observed band size : 48 kDa
Exposure time : 8 seconds

All lanes:

Western blot - Anti-Cyclin E1 (phospho T77) antibody [SD2025] (ab326630) at 1/1000 dilution

Lane 1:

K562 whole cell lysates at 20 µg

Lane 2:

K562 treated with 100nM Calyculin A for 30min whole cell lysates at 20 µg

Lane 3:

293T whole cell lysates at 20 µg

Lane 4:

293T treated with 100nM Calyculin A for 30min whole cell lysates at 20 µg

Lane 5:

293T treated with Lambda Protein Phosphatase for 30min whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 48 kDa

false

Exposure time: 8s

Western blot - Anti-Cyclin E1 (phospho T77) antibody [SD2025] (AB326630)
  • WB

Supplier Data

Western blot - Anti-Cyclin E1 (phospho T77) antibody [SD2025] (AB326630)

All lanes : Primary antibody at 1/1000 dilution
Lane 1 : K562 whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 47 kDa
Observed band size : 48 kDa
Exposure time : 15 seconds

All lanes:

Western blot - Anti-Cyclin E1 (phospho T77) antibody [SD2025] (ab326630) at 1/1000 dilution

Lane 1:

K562 whole cell lysates at 20 µg

Lane 2:

HepG2 whole cell lysates at 20 µg

Lane 3:

3T3 whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 48 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SD2025

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for the control of the cell cycle at the G1/S (start) transition.
See full target information CCNE1 phospho T77
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com