Rabbit Polyclonal Cyclin E2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CCNE2 aa 350 to C-terminus.
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Publications
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- International journal of molecular sciences 24:2022HMGA1 Regulates the Expression of Replication-Dependent Histone Genes and Cell-Cycle in Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Petrosino,Sabrina Pacor,Silvia Pegoraro,Virginia Anna Gazziero,Giulia Canarutto,Silvano Piazza,Guidalberto Manfioletti,Riccardo SgarraPubMed 36614035
- Translational cancer research 11:414-4252022Knockdown of inhibits the progression of ovarian cancer and is related to the signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFanling Meng,Jing Ding,Wei Xu,Chang Luo,Xihai Chen,Ruichun Zhang,Lin Sui,Yuanlong Hu,Shuang Liu,Guangyue Shi,Yunlong He,Xin Ning,Rong Ma,Ning HuangPubMed 35281418
- International journal of biological sciences 15:522-5322019Combined Androgen receptor blockade overcomes the resistance of breast cancer cells to palbociclib.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenfei Ji et. al.PubMed 30745839
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human CCNE2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O96020
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Essential for the control of the cell cycle at the late G1 and early S phase.
Alternative names
G1/S-specific cyclin-E2, CCNE2
Recommended products
Rabbit Polyclonal Cyclin E2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CCNE2 aa 350 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human CCNE2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O96020
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab226388 was affinity purified using an epitope specific to Cyclin E2 immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-Cyclin E2 antibody (ab226388)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-Cyclin E2 antibody (ab226388) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 3min
Immunoprecipitation - Anti-Cyclin E2 antibody (ab226388)
Cyclin E2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226388 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226388 at 0.4 μg/ml.
Lane 1: ab226388 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-Cyclin E2 antibody (ab226388)
Predicted band size: 47 kDa
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