Anti-Cyclin E2 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-Cyclin E2 antibody (AB226388)

Cyclin E2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226388 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226388 at 0.4 μg/ml.

Lane 1 : ab226388 IP in Jurkat whole cell lysate.

Lane 2 : Control IgG IP in Jurkat whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-Cyclin E2 antibody (ab226388)

Predicted band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-Cyclin E2 antibody (AB226388)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Cyclin E2 antibody (ab226388) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 47 kDa

true

Exposure time: 3min