Anti-Cyclin E2 antibody [E142] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] - BSA and Azide free (AB228478)

This data was developed using ab32103, the same antibody clone in a different buffer formulation.

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin E2 with purified ab32103 at 1/50 dilution (5.2 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• ICC/IF

AbReview60983****

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] - BSA and Azide free (AB228478)

ab32103 staining Cyclin E2 in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081 (1/200) was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32103).

This image is courtesy of an Abreview submitted by Kirk Mcmanus.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] - BSA and Azide free (AB228478)

This ICC data was generated using the same anti-Cyclin E2 antibody clone, E142, in a different buffer formulation (cat# ab32103).

Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling Cyclin E2 with purified ab32103 at 1/2000. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] - BSA and Azide free (AB228478)

Immunofluorescent analysis of Cyclin E2 expression in HeLa cell culture using 1/100 ab32103.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32103).

• WB

Lab

Western blot - Anti-Cyclin E2 antibody [E142] - BSA and Azide free (AB228478)

This data was developed using the same antibody clone in a different buffer formulation (ab32103)

All lanes:

Western blot - Anti-Cyclin E2 antibody [E142] (<a href='/en-us/products/primary-antibodies/cyclin-e2-antibody-e142-ab32103'>ab32103</a>) at 1/200 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Mouse testis lysate at 20 µg

Lane 3:

Mouse thymus lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 45 kDa

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