Anti-Cyclin E2 antibody [E142] - BSA and Azide free

5 product images

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  Western blot - Anti-Cyclin E2 antibody [E142] - BSA and Azide free (ab228478)

  This data was developed using the same antibody clone in a different buffer formulation (Anti-Cyclin E2 antibody [E142] ab32103)

  All lanes: Western blot - Anti-Cyclin E2 antibody [E142] (Anti-Cyclin E2 antibody [E142] ab32103) at 1/200 dilution

  Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: Mouse testis lysate at 20 µg

  Lane 3: Mouse thymus lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 47 kDa

  Observed band size: 45 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] - BSA and Azide free (ab228478)

  This data was developed using Anti-Cyclin E2 antibody [E142] ab32103, the same antibody clone in a different buffer formulation.
  
  Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin E2 with purified Anti-Cyclin E2 antibody [E142] ab32103 at 1/50 dilution (5.2 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] - BSA and Azide free (ab228478)

  This ICC data was generated using the same anti-Cyclin E2 antibody clone, E142, in a different buffer formulation (cat# Anti-Cyclin E2 antibody [E142] ab32103).
  

  Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling Cyclin E2 with purified Anti-Cyclin E2 antibody [E142] ab32103 at 1/2000. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

  Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

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  Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] - BSA and Azide free (ab228478)

  Immunofluorescent analysis of Cyclin E2 expression in HeLa cell culture using 1/100 Anti-Cyclin E2 antibody [E142] ab32103.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cyclin E2 antibody [E142] ab32103).

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  This image is courtesy of a customer review submitted by Kirk Mcmanus.

  Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] - BSA and Azide free (ab228478)

  Anti-Cyclin E2 antibody [E142] ab32103 staining Cyclin E2 in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (1/200) was used as the secondary antibody.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cyclin E2 antibody [E142] ab32103).